Methods for spatial analysis using RNA-templated ligation

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

PROFILING OF BIOLOGICAL ANALYTES WITH SPATIALLY BARCODED OLIGONUCLEOTIDE ARRAYS

This disclosure relates to methods for spatial profiling of analytes present in a biological sample. Also provided are methods for using spatially barcoded substrates to detect a biological analyte in a cell culture, an organism, and organoid. Also provided are methods for using spatially barcoded substrates to detect the temporal profile of a biological analyte.

Spatially encoded biological assays

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

INTERACTIVE ARTIFICIAL INTELLIGENCE-BASED RESPONSE LOOP TO A CYBERATTACK

An intelligent orchestration module can facilitate an AI augmented and adaptive interactive response loop between multiple AI-based engines. A cyber threat detection uses AI to detect a cyber threat. An autonomous response engine uses AI to mitigate the detected cyber threat. A cyber-security restoration engine uses AI to remediate nodes in the system to a trusted operational state. The prediction engine uses AI to conduct simulations of cyberattacks to assist in determining how a simulated cyberattack might occur in the system, and how to use the simulated cyberattack information to preempt possible escalations of an ongoing actual cyberattack. The multiple AI-based engines bilaterally exchange behavioral metrics between the AI-based engines to work together to provide an overall cyber threat response during the ongoing cyberattack in light of continuing attack activities and simulations of the cyberattack to predict what might occur in the nodes based on the mitigation actions taken and/or the restoration actions taken.

High and low pressure manifold liquid supply system for fracturing units

The present disclosure relates to a high and low pressure manifold fluid supply system for fracturing units, including: a trailer, a high and low pressure manifold arranged on the trailer, a support frame arranged on a platform of the trailer, and a power distribution switch cabinet arranged on the support frame, which is configured to be electrically connected to the electrically-driven fracturing units and configured to distribute electricity to the electrically-driven fracturing units. Through the high and low pressure manifold fluid supply system integrated with electricity supply facilities therein according to the present disclosure, the electrically-driven fracturing units are powered, in this way, the electricity supply and distribution system in the well site can be effectively simplified, the connection distance of the cables can be shorten, and further the time spent on connection can be saved, thereby improving the well site layout efficiency.

Cable Laying Device

The present application relates to a cable laying device (100). The cable laying device is used for laying a cable, and comprises: a cable laying part (10) comprising a base section (11) and an extension section (12); a turnover mechanism (20) and a positioning adjustment mechanism (30), wherein, the base section (11) and the extension section (12) are connected rotatably relative to each other at their longitudinal ends; the positioning adjustment mechanism (30) is connected to the base section and comprises a rotating assembly (31), wherein the rotating assembly (31) drives the cable laying part to rotate; and the turnover mechanism (20) connects the extension section (12) and the base section (11), and drives the extension section (12) to turn over relative to the base section (11).

FLEXIBLE PIPE END PORTION CONNECTION STRUCTURE

A flexible pipe end portion connection structure is provided. The flexible pipe end portion connection structure includes a flexible pipe body and a joint. The joint includes a first end and a second end opposite to each other and has a channel extending through the joint in a direction from the first end to the second end. An inner sidewall of the first end of the joint has a plurality of first stepped structures, and an inner diameter of a portion of the channel corresponding to the first stepped structure gradually decreases in the direction from the first end to the second end. At least a part of an end of the flexible pipe body is inserted into the first end of the joint to fit with the first stepped structure.

Computer implemented method for the automated analysis or use of data

A computer implemented method for the automated analysis or use of data is implemented by a voice assistant. The method comprises the steps of: (a) storing in a memory a structured, machine-readable representation of data that conforms to a machine-readable language (‘machine representation’); the machine representation including representations of user speech or text input to a human/machine interface; and (b) automatically processing the machine representations to analyse the user speech or text input.

Personal space bubble in VR environments

A system and method for providing automated intervention in a virtual environment is disclosed. Intervention settings for a user are stored, wherein the intervention settings for the user includes one or more customized intervention triggers each corresponding to one or more trigger conditions and an intervention response. A current session associated with a user device of the user is monitored, wherein monitoring the current session includes analyzing interactions between the user and one or more other users in a virtual environment of the current session based on the intervention settings. One of the intervention triggers is identified as having occurred in the current session based on detecting the corresponding trigger conditions in the virtual environment. A workflow associated with the intervention response that corresponds to the identified intervention trigger is modulated based on the interactions between the user and the other users in the virtual environment. The intervention response is executed in the virtual environment of the current session based on the modulated workflow.

DETECTING VULNERABILITIES IN CONFIGURATION CODE OF A CLOUD ENVIRONMENT UTILIZING INFRASTRUCTURE AS CODE

A system and method for applying a unified security policy across a technology stack, includes detecting a cloud object in a first cloud computing environment, the cloud object including a plurality of attributes, each attribute having a corresponding value; detecting a node in a security graph having a data field value which matches an attribute value of the cloud object, wherein the security graph includes a representation of a cloud environment; applying a policy based on the data field value to the detected cloud object; and applying the policy to another cloud object in a second cloud computing environment, in response to determining that a node representing the cloud object in the security graph is connected to a node representing the another cloud object.