-
2006-04-25
10/493,682
2002-10-10
US 7,033,626 B2
2006-04-25
WO; PCT/DK02/00641; 20021010
WO; WO03/034838; 20030501
Keith Hendricks
2022-10-10
In the production of edible products which includes a holding period for starch retrogra-dation, the addition of a xylanase to cereal-based raw materials accelerates the retrogradation and thus allows a shortening of the holding period.
Get notified when new applications in this technology area are published.
A21D8/04 IPC
Methods for preparing or baking dough; Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
This application is a 35 U.S.C. 371 national application of PCT/DK02/00641, filed Oct. 10, 2002, which claims priority or the benefit under 35 U.S.C. 119 of Danish application nos. PA 2001 01574, filed Oct. 26, 2001, and PA 2002 00051, filed Jan. 14, 2002, and U.S. provisional application No. 60/347,574, filed Jan. 11, 2002, the contents of which are fully incorporated herein by reference.
The present invention relates to the preparation of an edible product from cereal-based raw materials and water by heating, cooling, holding and drying.
The preparation of some cereal-based food products includes a holding step (sometimes known as tempering or ageing) intended to make the starch retrograde sufficiently to make the product acceptable for further processing. This may typically involve holding from 10 to 48 hours at room temperature.
An example is the production of certain snack products (sometimes called third-generation or 3G snacks) in a process including extrusion cooking, followed by cooling, holding and drying to make snack pellets which are expanded by heating (e.g. by frying In oil) to make the final snack product.
Another example is shredded cereals made by cooking whole grain (particularly wheat), followed by cooling, tempering, shredding, forming into biscuits and baking.
The ageing step is by nature a time and space consuming step, and a shortening of this step will give significant advantages to manufactures such as the possibility of reducing floor and rack space or increase production.
The inventors have found that in the production of edible products which includes a holding period for starch retrogradation, the addition of a xylanase to cereal-based raw materials accelerates the retrogradation and thus allows a shortening of the holding period.
Accordingly, the invention provides a process for producing an edible product, comprising the following sequential steps:
a) mixing a xylanase with cereal-based raw materials,
b) heating so as to gelatinize starch,
c) cooling,
d) holding to effect retrogradation of the starch, and
e) heating.
Food Product
The food product may be snack pellets, a snack product (e.g. a third-generation snack), or shredded cereal (e.g. shredded wheat) for use as a breakfast cereal.
Snack Pellets and Products
According to the invention, snack pellets may be produced by a process comprising the following sequential steps:
a) mixing a xylanase with cereal-based raw materials,
b) extruding and heating the mixture so as to gelatinize starch,
c) cooling and holding to effect retrogradation of the starch, and
d) heating and drying.
The raw material mixture typically contains up to 32% water (e.g. 20β32%), and may optionally be preconditioned by heating, e.g. up to 95Β° C. for 20β240 seconds.
The extrusion cooking may be done in a single-screw or double-screw extruder with a residence time of 30β90 seconds. The extruder will typically comprise a cooking zone at 80β150Β° C. and a forming zone at 65β90Β° C. After the extrusion with heating, the mixture will be formed into long rods, typically having a temperature of 60β100Β° C. (particularly 70β95Β° C.) and a moisture content of 25β30% or 20β28%. The holding (also termed aging) may take 8β24 hours (particularly 10β16 hours). Before or during the holding, the rods will be cooled, typically to 15β30Β° C. The holding serves to affect at least partial retrogradation of the starch, either of the amylose component, the amylopectin component or both. Advantageously, the xylanase added according to the invention accelerates the retrogradation, so the holding time may be shortened. The end-point is conventionally determined by testing the hardness and brittleness of the pellets.
After ageing the rods are cut into pellets.
The drying of the pellets may be done at 70β95Β° C. for 1β3 hours to reach an exit moisture of 6β8% in the snack pellets.
The dried snack pellets may be stored or distributed to a snack processor. The snack pellets may then be expanded by heating, e.g. by frying in oil or puffing in hot air or in microwave or infrared oven.
Shredded Cereals
According to the invention, shredded cereals may be produced by a process comprising the following sequential steps:
a) mixing a xylanase with cereal-based raw materials,
b) cooking so as to gelatinize starch,
c) cooling and holding to effect retrogradation of the starch,
e) shredding, and
f) baking.
Shredded cereals may be made by cooking whole grain, followed by cooling, tempering, shredding, forming into biscuits and baking.
The whole grain may be wheat (e.g. white wheat), rice or corn. The cooking may be done for 30β35 minutes at atmospheric pressure or 2000 hPa to reach 45β50% moisture after removal of excess water. The holding (or tempering) may take 8β28 hours with cooling to 15β30Β° C. After shredding, the shreds may be stacked to make a biscuit, and this may be baked at 200β315Β° C. to around 4% end moisture.
Xylanase
The invention uses a xylanase, i.e. an enzyme having the activity classified as EC 3.2.1.8 according to Enzyme Nomenclature. The enzyme may suitably have a pH optimum in the range 5β9.
The xylanase may have hydrolytic activity on arabinoxylan with a high specificity for soluble arabinoxylan compared to Insoluble arabinoxylan. The specificity may be expressed as WSPS defined as the ratio WSP/WIP described in WO 9523514; the xylanase may have WSPS above 0.1, e.g. above 1.0 or above 2.5. Particular examples are Xylanases I and II from A. aculeatus described in WO 9523514.
The enzyme may suitably have a temperature optimum in the presence of starch in the range of 30β90Β° C., preferably 50β80Β° C., particularly 55β75Β° C., e.g. 60β70Β° C. The temperature optimum may be measured in a 1% solution of soluble starch at pH 5.5.
The enzyme is typically used at a dosage of 0.1β20 mg enzyme protein per kg of dry solids in the raw material, particularly 0.5β5 mg/kg.
The xylanase may be of any origin including mammalian, plant or animal origin, e.g. of microbial origin. In particular the xylanase preparation may be derived from a filamentous fungus or a yeast. More particularly, the xylanase may be derived from a strain of the following genus or species: Aspergillus, A. niger, A. awamori, A. aculeatus, A. oryzae, A. tubigensis, Trichoderma, T. reesei, T. harzianum, Penicillium, P. camenbertil, Fusarium, F. oxysporum, Thermomyces, T. lanuginosus, Humicola, H. insolens, Bacillus, B. pumilus.
Rod-type snack pellets were produced with addition of xylanase. The xylanase was Shearzyme, a purified xylanase from Aspergillus aculeatus produced by a genetically modified strain of Aspergillus oryzae at a dosage of 150 FXU/kg of raw material (the FXU xylanase activity unit is defined in |WO 9404035|). A control was made without addition of xylanase.
The following raw materials were mixed: Potato starch, wheat flour, maize flour, potato granules, salt, paprika, vegetable oil, emulsifier and flavouring.
The raw materials were treated by pre-conditioning at 20β95Β° C. for 1β2 minutes followed by extrusion at 80β150Β° C. for 30β90 seconds, and forming at 65β90Β° C. into long rods.
After forming the rods were placed on racks and stored 16β24 hours before cutting. At the start of ageing the rods were rubber like and did not break when bended. A simple bending test was performed several times during storage, and the rods were judged to be ready for cutting when they readily broke at the bend point when bended.
Finally, the rods were cut and expanded by deep-frying in palm oil at 180Β° C. for 9β11 seconds.
The results were that the rods made with the xylanase were ready after 18β19 hours whereas the control without endo-amylase was ready after 24 hours. The expanded pellets from the enzyme trials did not show any significant differences compared to the reference on physical parameters.
Snack pellets were produced according to a sheeted pellet procedure with addition of xylanase. A control was made without xylanase.
The following raw materials were mixed: Potato granules, glucose, salt, vegetable oil, mono- and diglycerides as emulsifiers, and dicalciumphosphate.
The raw materials were treated by preconditioning at 20β80Β° C. for 1β2 minutes, followed by extrusion at 80β130Β° C. for 30β45 seconds, forming (sheeting) and drying of the single pellets. The pellets were allowed to rest for at least 24 hours to assure optimal water migration, before expansion. Expansion was done In palm oil at approximately 180Β° C. for 9β11 seconds.
The texture was judged by a panel of 4 persons. The xylanase, the dosage used and the observed effect were as follows:
| Dosage/kg | ||
| Enzyme | raw material | Effect |
| Xylanase from | 2000 FXU | A little firm. No big differences between |
| Thermomyces | reference and sample | |
| lanuginosus | 5000 FXU | A little firm. No big differences between |
| reference and sample. A little stronger | ||
| in taste compared to reference, but no | ||
| bad taste. A very nice product. | ||
All enzyme-treated products looked nicer than the reference with fewer, smaller and better distributed air bubbles after expansion.
1. A process for producing an edible product, comprising the following sequential steps:
a) mixing a xylanase with cereal-based raw materials comprising starch,
b) heating so as to gelatinize the starch,
c) cooling and holding the starch to effect retrogradation of the starch, and
d) heating and drying the starch,
wherein the xylanase accelerates the retrogradation and the holding period is shorter than that required of the same process without added xylanase.
2. The process of claim 1, which further comprises after said step (d), frying the starch in oil.
3. The process of claim 1, wherein the edible product is a snack food or a breakfast cereal.
4. The process of claim 2, wherein the edible product is a snack food or a breakfast cereal.
5. The process of claim 1, which further comprises after said step (d), puffing the starch in hot air.
6. The process of claim 1, which further comprises after said step (d), heating the starch in a microwave oven.
7. The process of claim 1, which further comprises after said step (d), heating the starch in an infrared oven.
8. A process for producing snack pellets, comprising the following sequential steps:
a) mixing a xylanase with cereal-based raw materials comprising starch,
b) heating and extruding the mixture so as to gelatinize the starch and form pellets,
c) cooling and holding the pellets to effect retrogradation of the starch, and
d) heating and drying the pellets,
wherein the xylanase accelerates the retrogradation and the holding period is shorter than that required of the same process without added xylanase.
9. The process of claim 8, comprising, following said step (d), frying the pellets in oil.
10. The process of claim 8, which further comprises after said step (d), puffing the starch in hot air.
11. The process of claim 8, which further comprises after said step (d), heating the starch in a microwave oven.
12. The process of claim 8, which further comprises after said step (d), heating the starch in an infrared oven.
13. A process for producing shredded cereals, comprising the following sequential steps:
a) mixing a xylanase with cereal-based raw materials comprising starch,
b) cooking the starch so as to gelatinize the starch,
c) cooling and holding the starch to effect retrogradation of the starch,
d) shredding the starch, and
e) baking the starch,
wherein the xylanase accelerates the retrogradation and the holding period is shorter than that required of the same process without added xylanase.