GENERATION OF HIGH POLYHYDROXYBUTRATE PRODUCING OILSEEDS

Description

FIELD OF THE INVENTION

The invention is in the field of polymer production in transgenic plants. Methods for generating industrial oilseeds producing high levels of polyhydroxybutyrate (PHB) and industrial oilseeds producing high levels of PHB are described.

BACKGROUND OF THE INVENTION

Production of polyhydroxyalkanoates (PHAs), a family of naturally occurring renewable and biodegradable plastics, in crops has the potential of providing a renewable source of polymers, chemical intermediates and bio-energy from one crop if plant residues remaining after polymer isolation are converted to liquid fuels and/or energy. PHAs can provide an additional revenue stream that would make bioenergy crops more economically viable.

PHAs are a natural component of numerous organisms in multiple ecosystems and accumulate in a wide range of bacteria as a granular storage material when the microbes are faced with an unfavorable growth environment, such as a limitation in an essential nutrient (Madison et al., Microbiol. Mol. Biol. Rev., 1999, 63, 21-53; Suriyamongkol et al., Biotechnol Adv, 2007, 25, 148-175). The monomer unit composition of these polymers is largely dictated by available carbon source as well as the native biochemical pathways present in the organism. Today PHAs are produced industrially from renewable resources in bacterial fermentations providing an alternative to plastics derived from fossil fuels. PHAs possess properties enabling their use in a variety of applications currently served by petroleum-based plastics and are capable of matching or exceeding the performance characteristics of fossil fuel derived plastics with a broad spectrum of properties that can be obtained by varying the monomer composition of homo- and co-polymers, or by manipulating properties such as molecular weight (Sudesh et al., Prog. Polym. Sci., 2000, 25, 1503-1555; Sudesh et al., CLEAN—Soil, Air, Water, 2008, 36, 433-442).

Industrial production of PHAs in crop plants would provide a low cost, renewable source of plastics. Production of PHAs in plants has been an as yet unsolved goal for plant scientists and has previously been demonstrated in a number of crops unsuitable for industrial production or in industrially useful crops at levels to low to be commercially attractive [for review, see (Suriyamongkol et al., Biotechnol Adv, 2007, 25, 148-175); (van Beilen et al., The Plant Journal, 2008, 54, 684-701) and references within] including maize (Poirier et al., 2002, Polyhydroxyalkanoate production in transgenic plants, in Biopolymers, Vol 3a, Steinbuchel, A. (ed), Wiley-VHC Verlag GmbH, pgs 401-435), sugarcane (Purnell et al., Plant Biotechnol. J., 2007, 5, 173-184), switchgrass (Somleva et al., Plant Biotechnol J, 2008, 6, 663-678), flax (Wrobel et al., J. Biotechnol., 2004, 107, 41-54; Wrobel-Kwiatkowsk et al., Biotechnol Prog, 2007, 23, 269-277), cotton (John et al., Proceedings of the National Academy of Sciences of the United States of America, 1996, 93, 12768-12773), alfalfa (Saruul et al., Crop Sci., 2002, 42, 919-927), tobacco (Arai et al., Plant Biotechnol., 2001, 18, 289-293; Bohmert et al., Plant Physiol., 2002, 128, 1282-1290; Lossl et al., Plant Cell Reports, 2003, 21, 891-899; Lössl et al., Plant Cell Physiol, 2005, 46, 1462-1471), potato (Bohmert et al., Plant Physiol., 2002, 128, 1282-1290), and oilseed rape (Valentin et al., Int. J. Biol. Macromol., 1999, 25, 303-306; Slater et al., Nat. Biotechnol., 1999, 17, 1011-1016). Most of the efforts to produce PHAs in plants have focused on production of the homopolymer P3HB or the copolymer poly-3-hydroxybutyrate-co-3-hydroxyvalerate (P3HBV). While there have been some efforts to produce medium chain length PHAs in plants, these studies have yielded barely detectable levels of polymer (Romano et al., Planta, 2005, 220, 455-464; Mittendorf et al., Proceedings of the National Academy of Sciences of the United States of America, 1998, 95, 13397-13402; Poirier et al., Plant Physiol., 1999, 121, 1359-1366; Matsumoto, Journal of Polymers and the Environment, 2006, 14, 369-374; Wang et al., Chinese Science Bulletin, 2005, 50, 1113-1120).

To date, the highest levels of polymer have been obtained when the homopolymer poly-3-hydroxybutyrate (P3HB or PHB) is produced in plastids (Suriyamongkol et al., Biotechnol Adv, 2007, 25, 148-175; van Beilen et al., The Plant Journal, 2008, 54, 684-701; Bohmert et al., Molecular Biology and Biotechnology of Plant Organelles, 2004, 559-585). This is likely due to the high flux of acetyl-CoA, the precursor for PHB in these organelles during fatty acid biosynthesis (Bohmert et al, Molecular Biology and Biotechnology of Plant Organelles, 2004, 559-585). Expression of three genes encoding β-ketothiolase, acetoacetyl CoA reductase, and PHA synthase, allows the conversion of acetyl-CoA within the plastid to PHB. Previous work has reported producing levels of PHB in Brassica napus up to a maximum of 6.7% of seed weight, a level too low for commercial production

SUMMARY OF THE INVENTION

Transgenic oilseed plants, plant material, plant cells, and genetic constructs for synthesis of polyhydroxyalkanoates (“PHA”) are provided. In a preferred embodiment, the transgenic oilseed plants synthesize (poly)3-hydroxybutyrate (“PHB”) in the seed. Host plants, plant tissue, and plant material have been engineered to express genes encoding enzymes in the biosynthetic pathway for PHB production such that polymer precursors in the plastid are polymerized to polymer. Genes utilized include phaA, phaB, phaC, all of which are known in the art. The genes can be introduced in the plant, plant tissue, or plant cell using conventional plant molecular biology techniques.

It is an object of the invention to provide methods and compositions for producing transgenic oilseeds having commercially viable levels of polyhydroxyalkanoates in the seed, for example greater than 7%, 10%, 15%, or 19% polyhydroxyalkanoate or more of the total dry seed weight.

It is another object of the invention to provide oilseeds having increased levels of polyhydroxyalkanoate greater than 7%, 10%, 15%, or 19% polyhydroxyalkanoate or more of the total dry seed weight and having impaired germination relative to non-transgenic oilseeds.

Using a non-traditional screening method to identify transgenic lines than those used in all other reported studies, it has been discovered that very high levels of PHA, for example PHB can be produced in the oilseed but that oilseeds with high levels of PHA fail to germinate or germinate but produce impaired seedlings which do not survive to produce viable fertile plants. The failure to produce viable progeny explains why previous researchers failed to demonstrate that commercial levels of PHA can be produced in transgenic oilseeds. A preferred PHA produced in oilseeds is PHB.

In another embodiment the transgenes encoding PHA biosynthesis genes are expressed in a seed specific manner such that the PHA accumulates in the seed. In this embodiment the level of PHA accumulated is greater than 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18% and 19% of the dry weight of the seed.

Methods and compositions for producing hybrid lines are also provided. Hybrid lines can be created by crossing a line containing one or more PHAs, for example PHB genes with a line containing the other gene(s) needed to complete the PHA biosynthetic pathway. Use of lines that possess cytoplasmic male sterility with the appropriate maintainer and restorer lines allows these hybrid lines to be produced efficiently.

In still another embodiment the oilseeds produced by the disclosed methods produce high levels of PHA and are impaired in their ability to germinate and survive to produce viable plants relative to oilseeds containing little or no PHA, for example less than 7% PHA of the dry weight of the seed. Germination can be impaired by 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100% relative to oilseeds with less than 7% PHA. Impaired germination provides a built in mechanism for gene containment reducing the risk of unwanted growth of these oilseeds when a different crop is planted on the production fields.

Transgenic plants useful for the invention include dicots or monocots. Preferred host plants are oilseed plants, but are not limited to members of the Brassica family including B. napus, B. rapa, B. carinata and B. juncea. Additional preferred host plants include industrial oilseeds such as Camelina sativa, Crambe, jatropha, and castor. Other preferred host plants include Arabidopsis thaliana, Calendula, Cuphea, maize, soybean, cottonseed, sunflower, palm, coconut, safflower, peanut, mustards including Sinapis alba, and tobacco.

Other embodiments provide plant material and plant parts of the transgenic plants including seeds, flowers, stems, and leaves. The oilseeds can be used for the extraction of PHA biopolymer or as a source of PHA biopolymer based chemical intermediates. The residual parts of the seed can be used as meal for animal feed or steam and power generation and a source of vegetable oil for industrial oelochemicals or biofuel.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a schematic diagram describing a strategy for creating hybrid seeds using cytoplasmic male sterility.

DETAILED DESCRIPTION OF THE INVENTION

I. Definitions

Unless otherwise indicated, the disclosure encompasses all conventional techniques of plant breeding, microbiology, cell biology and recombinant DNA, which are within the skill of the art. See, e.g., Sambrook and Russell, Molecular Cloning: A Laboratory Manual, 3rd edition (2001); Current Protocols In Molecular Biology [(F. M. Ausubel, et al. eds., (1987)]; Plant Breeding Principles and Prospects (Plant Breeding, Vol 1) M. D. Hayward, N. O. Bosemark, I. Romagosa; Chapman & Hall, (1993.); Coligan, Dunn, Ploegh, Speicher and Wingfeld, eds. (1995) Current Protocols in Protein Science (John Wiley & Sons, Inc.); the series Methods in Enzymology (Academic Press, Inc.): PCR 2: A Practical Approach (M. J. MacPherson, B. D. Hames and G. R. Taylor eds. (1995)].

Unless otherwise noted, technical terms are used according to conventional usage. Definitions of common terms in molecular biology may be found in Lewin, Genes VII, published by Oxford University Press, 2000; Kendrew et al. (eds.), The Encyclopedia of Molecular Biology, published by Wiley-Interscience., 1999; and Robert A. Meyers (ed.), Molecular Biology and Biotechnology, a Comprehensive Desk Reference, published by VCH Publishers, Inc., 1995; Ausubel et al. (1987) Current Protocols in Molecular Biology, Green Publishing; Sambrook and Russell. (2001) Molecular Cloning: A Laboratory Manual 3rd. edition.

A number of terms used herein are defined and clarified in the following section.

The term PHB refers to polyhydroxybutyrate and is used interchangeably with the term PHA which refers to polyhydroxyalkanoate.

The tend PHB also encompasses copolymers of hydroxybutyrate with other hydroxyacid monomers.

The term “PHA copolymer” refers to a polymer composed of at least two different hydroxyalkanoic acid monomers.

The term “PHA homopolymer” refers to a polymer that is composed of a single hydroxyalkanoic acid monomer.

As used herein, a “vector” is a replicon, such as a plasmid, phage, or cosmid, into which another DNA segment may be inserted so as to bring about the replication of the inserted segment. The vectors can be expression vectors.

As used herein, an “expression vector” is a vector that includes one or more expression control sequences

As used herein, an “expression control sequence” is a DNA sequence that controls and regulates the transcription and/or translation of another DNA sequence. Control sequences that are suitable for prokaryotes, for example, include a promoter, optionally an operator sequence, a ribosome binding site, and the like. Eukaryotic cells are known to utilize promoters, polyadenylation signals, and enhancers.

As used herein, “operably linked” means incorporated into a genetic construct so that expression control sequences effectively control expression of a coding sequence of interest.

As used herein, “transformed” and “transfected” encompass the introduction of a nucleic acid into a cell by a number of techniques known in the art.

“Plasmids” are designated by a lower case “p” preceded and/or followed by capital letters and/or numbers.

As used herein the term “heterologous” means from another host. The other host can be the same or different species.

The term “cell” refers to a membrane-bound biological unit capable of replication or division.

The term “construct” refers to a recombinant genetic molecule including one or more isolated polynucleotide sequences.

Genetic constructs used for transgene expression in a host organism comprise in the 5′-3′ direction, a promoter sequence; a nucleic acid sequence encoding the desired transgene product; and a termination sequence. The open reading frame may be orientated in either a sense or anti-sense direction. The construct may also comprise selectable marker gene(s) and other regulatory elements for expression.

The term “plant” is used in it broadest sense. It includes, but is not limited to, any species of woody, ornamental or decorative, crop or cereal, fruit or vegetable plant, and photosynthetic green algae (e.g., Chlamydomonas reinhardtii). It also refers to a plurality of plant cells that are largely differentiated into a structure that is present at any stage of a plant's development. Such structures include, but are not limited to, a fruit, shoot, stem, leaf, flower petal, etc. The term “plant tissue” includes differentiated and undifferentiated tissues of plants including those present in roots, shoots, leaves, pollen, seeds and tumors, as well as cells in culture (e.g., single cells, protoplasts, embryos, callus, etc.). Plant tissue may be in planta, in organ culture, tissue culture, or cell culture. The term “plant part” as used herein refers to a plant structure, a plant organ, or a plant tissue.

A non-naturally occurring plant refers to a plant that does not occur in nature without human intervention. Non-naturally occurring plants include transgenic plants and plants produced by non-transgenic means such as plant breeding.

The term “plant cell” refers to a structural and physiological unit of a plant, comprising a protoplast and a cell wall. The plant cell may be in form of an isolated single cell or a cultured cell, or as a part of higher organized unit such as, for example, a plant tissue, a plant organ, or a whole plant.

The term “plant cell culture” refers to cultures of plant units such as, for example, protoplasts, cell culture cells, cells in plant tissues, pollen, pollen tubes, ovules, embryo sacs, zygotes and embryos at various stages of development.

The term “plant material” refers to leaves, stems, roots, flowers or flower parts, fruits, pollen, egg cells, zygotes, seeds, cuttings, cell or tissue cultures, or any other part or product of a plant.

A “plant organ” refers to a distinct and visibly structured and differentiated part of a plant such as a root, stem, leaf, flower bud, or embryo.

“Plant tissue” refers to a group of plant cells organized into a structural and functional unit. Any tissue of a plant, whether in a plant or in culture, is included. This term includes, but is not limited to, whole plants, plant organs, plant seeds, tissue culture and any groups of plant cells organized into structural and/or functional units. The use of this term in conjunction with, or in the absence of, any specific type of plant tissue as listed above or otherwise embraced by this definition is not intended to be exclusive of any other type of plant tissue.

“Seed germination” refers to growth of an embryonic plant contained within a seed resulting in the formation and emergence of a seedling.

“Cotyledon” refers to the embryonic first leaves of a seedling.

“Early plantlet development” refers to growth of the cotyledon containing seedling to form a plantlet.

II. Transgenic Plants

Transgenic plants have been developed that produce increased levels of biopolymers such as polyhydroxyalkanoates (PHAs) in seeds. Methods and constructs for engineering plants for seed specific production of PHA, in particular PHB, are described. One embodiment provides transgenic plants for the direct, large scale production of PHAs in crop plants or in energy crops where a plant by-product, such as oil, can be used for production of energy. Proof of concept studies for polyhydroxybutyrate (PHB) synthesis in canola (Valentin et al., Int. J. Biol. Macromol., 1999, 25, 303-306; Houmiel et al., Planta, 1999, 209, 547-550; Slater et al., Nat. Biotechnol., 1999, 17, 1011-1016.) have been reported. There have been instances where high level PHB production in the chloroplasts of plants has led to decreases in total plant growth (Bohmert et al., Molecular Biology and Biotechnology of Plant Organelles, 2004, 559-585; Bohmert et al., Planta, 2000, 211, 841-845) for unidentified reasons. There have been several studies that have attempted to alleviate this problem by inducible expression of enzymes (Bohmert et al., Plant Physiol., 2002, 128, 1282-1290; Lössl et al., Plant Cell Physiol, 2005, 46, 1462-1471; Kourtz et al., Transgenic Res, 2007, 16, 759-769).

Transgenic oilseeds comprising at least about 8% dry weight PHA are provided. In one embodiment we provide transgenic oilseeds having at least 10% PHA dry weight and which are impaired in germination and plant survival.

A. Genetic Constructs for Transformation

Suitable genetic constructs include expression cassettes for enzymes for production of polyhydroxyalkanoates, in particular from the polyhydroxybutyrate biosynthetic pathway. In one embodiment, the construct contains operatively linked in the 5′ to 3′ direction, a seed specific promoter that directs transcription of a nucleic acid sequence in the nucleus; a nucleic acid sequence encoding one of the PHB biosynthetic enzymes; and a 3′ polyadenylation signal that increases levels of expression of transgenes. In one embodiment, enzymes for formation of polymer precursors are targeted to the plastid using appropriate plastid-targeting signals. In another embodiment, the PHA pathway is expressed directly from the plastid genome using appropriate plastidial promoters and regulatory sequences.

DNA constructs useful in the methods described herein include transformation vectors capable of introducing transgenes into plants. As used herein, “transgenic” refers to an organism in which a nucleic acid fragment containing a heterologous nucleotide sequence has been introduced. The transgenes in the transgenic organism are preferably stable and inheritable. The heterologous nucleic acid fragment may or may not be integrated into the host genome.

Several plant transformation vector options are available, including those described in “Gene Transfer to Plants” (Potrykus, et al., eds.) Springer-Verlag Berlin Heidelberg New York (1995); “Transgenic Plants: A Production System for Industrial and Pharmaceutical Proteins” (Owen, et al., eds.) John Wiley & Sons Ltd. England (1996); and “Methods in Plant Molecular Biology: A Laboratory Course Manual” (Maliga, et al. eds.) Cold Spring Laboratory Press, New York (1995). Plant transformation vectors generally include one or more coding sequences of interest under the transcriptional control of 5′ and 3′ regulatory sequences, including a promoter, a transcription termination and/or polyadenylation signal, and a selectable or screenable marker gene. For the expression of two or more polypeptides from a single transcript, additional RNA processing signals and ribozyme sequences can be engineered into the construct (U.S. Pat. No. 5,519,164). This approach has the advantage of locating multiple transgenes in a single locus, which is advantageous in subsequent plant breeding efforts.

Engineered minichromosomes can also be used to express one or more genes in plant cells. Cloned telomeric repeats introduced into cells may truncate the distal portion of a chromosome by the formation of a new telomere at the integration site. Using this method, a vector for gene transfer can be prepared by trimming off the arms of a natural plant chromosome and adding an insertion site for large inserts (Yu et al., Proc Natl Acad Sci USA, 2006, 103, 17331-6; Yu et al., Proc Natl Acad Sci USA, 2007, 104, 8924-9). The utility of engineered minichromosome platforms has been shown using Cre/lox and FRT/FLP site-specific recombination systems on a maize minichromosome where the ability to undergo recombination was demonstrated (Yu et al., Proc Natl Acad Sci USA, 2006, 103, 17331-6; Yu et al., Proc Natl Acad Sci USA, 2007, 104, 8924-9). Such technologies could be applied to minichromosomes, for example, to add genes to an engineered plant. Site specific recombination systems have also been demonstrated to be valuable tools for marker gene removal (Kerbach, S. et al., Theor Appl Genet, 2005, 111, 1608-1616), gene targeting (Chawla, R. et al., Plant Biotechnol J, 2006, 4, 209-218; Choi, S. et al., Nucleic Acids Res, 2000, 28, E19; Srivastava, V, & Ow, D W, Plant Mol Biol, 2001, 46, 561-566; Lyznik, L A, et al., Nucleic Acids Res, 1993, 21, 969-975), and gene conversion (Djukanovic, V, et al., Plant Biotechnol J, 2006, 4, 345-357).

An alternative approach to chromosome engineering in plants involves in vivo assembly of autonomous plant minichromosomes (Carlson et al., PLoS Genet, 2007, 3, 1965-74). Plant cells can be transformed with centromeric sequences and screened for plants that have assembled autonomous chromosomes de novo. Useful constructs combine a selectable marker gene with genomic DNA fragments containing centromeric satellite and retroelement sequences and/or other repeats.

Another approach is Engineered Trait Loci (“ETL”) technology (U.S. Pat. No. 6,077,697 to Hadlaczky et al.; US Patent Application 2006/0143732). This system targets DNA to a heterochromatic region of plant chromosomes, such as the pericentric heterochromatin, in the short arm of acrocentric chromosomes. Targeting sequences may include ribosomal DNA (rDNA) or lambda phage DNA. The pericentric rDNA region supports stable insertion, low recombination, and high levels of gene expression. This technology is also useful for stacking of multiple traits in a plant (US Patent Application 2006/0246586, 2010/0186117 and PCT WO 2010/037209).

Zinc-finger nucleases (ZFNs) are also useful in that they allow double strand DNA cleavage at specific sites in plant chromosomes such that targeted gene insertion or deletion can be performed (Shukla et al., Nature, 2009; Townsend et al., Nature, 2009).

For direct expression of transgenes from the plastid genome, a vector to transform the plant plastid chromosome by homologous recombination (as described in U.S. Pat. No. 5,545,818 to McBride et al.) is used in which case it is possible to take advantage of the prokaryotic nature of the plastid genome and insert a number of transgenes as an operon. WO 2010/061186 describes an alternative method for introducing genes into the plastid chromosome using an adapted endogenous cellular process for the transfer of RNAs from the cytoplasm to the plastid where they are incorporated by homologous recombination. This plastid transformation procedure is also suitable for practicing the disclosed compositions and methods.

A transgene may be constructed to encode a multifunctional enzyme through gene fusion techniques in which the coding sequences of different genes are fused with or without linker sequences to obtain a single gene encoding a single protein with the activities of the individual genes. Transgenes encoding a bifunctional protein containing thiolase and reductase activities (Kourtz, L., K. et al. (2005), Plant Biotechnol. 3: 435-447) and a trifunctional protein having each of the three enzyme activities required for PHB expression in plants (Mullaney and Rehm (2010), Journal of Biotechnology 147: 31-36) have been described. Such synthetic fusion gene/enzyme combinations can be further optimized using molecular evolution technologies.

A transgene may be constructed to encode a series of enzyme activities separated by intein sequences such that on expression, two or more enzyme activities are expressed from a single promoter as described by Snell in U.S. Pat. No. 7,026,526 to Metabolix, Inc.

1. Genes Involved in Polyhydroxyalkanoate Synthesis

In a preferred embodiment, the products of the transgenes are enzymes and other factors required for production of a biopolymer, such as a polyhydroxyalkanoate (PHA).

For PHA production, transgenes encode enzymes such as beta-ketothiolase, acetoacetyl-CoA reductase, PHB (“short chain”) synthase, PHA (“long chain”) synthase, threonine dehydratase, dehydratases such as 3-OH acyl ACP, isomerases such as Δ 3-cis, Δ 2-trans isomerase, propionyl-CoA synthetase, hydroxyacyl-CoA synthetase, hydroxyacyl-CoA transferase, R-3-hydroxyacyl-ACP:CoA transferase, thioesterase, fatty acid synthesis enzymes and fatty acid beta-oxidation enzymes. Useful genes are well known in the art, and are disclosed for example by Snell and Peoples Metab. Eng. 4: 29-40 (2002); Bohmert et. al. in Molecular Biology and Biotechnology of Plant Organelles. H. Daniell, C. D. Chase Eds., Kluwer Academic Publishers, Netherlands, 2004, pp. 559-585; (Suriyamongkol et al., Biotechnol Adv, 2007, 25, 148-175; van Beilen et al., The Plant Journal, 2008, 54, 684-701).

PHA Synthases

Examples of PHA synthases include a synthase with medium chain length substrate specificity, such as phaC1 from Pseudomonas oleovorans (WO 91/000917; Huisman, et al. J. Biol. Chem. 266, 2191-2198 (1991)) or Pseudomonas aeruginosa (Timm, A. & Steinbuchel, A. Eur. J. Biochem. 209: 15-30 (1992)), the synthase from Alcaligenes eutrophus with short chain length specificity (Peoples, O. P. & Sinskey, A. J. J. Biol. Chem. 264:15298-15303 (1989)), or a two subunit synthase such as the synthase from Thiocapsa pfennigii encoded by phaE and phaC (U.S. Pat. No. 6,011,144). Other useful PHA synthase genes have been isolated from, for example, Alcaligenes latus (Accession ALU47026), Burkholderia sp. (Accession AF153086), Aeromonas caviae (Fukui & Doi, J. Bacteriol. 179: 4821-30 (1997)), Acinetobacter sp. strain RA3849 (Accession L37761), Rhodospirillum rubrum (U.S. Pat. No. 5,849,894), Rhodococcus ruber (Pieper & Steinbuechel, FEMS Microbiol. Lett. 96(1): 73-80 (1992)), and Nocardia corallina (Hall et. al., Can. J. Microbiol. 44: 687-91 (1998)), Arthrospira sp. PCC 8005 (Accessions ZP_—07166315 and ZP_—07166316), Cyanothece sp. PCC 7425 (Accessions ACL46371 and ACL46370) and Synechocystis sp. PCC6803 (Accession BAA17430; Hein et al. (1998), Archives of Microbiology 170: 162-170).

PHA synthases with broad substrate specificity useful for producing copolymers of 3-hydroxybutyrate and longer chain length (from 6 to 14 carbon atoms) hydroxyacids have also been isolated from Pseudomonas sp. A33 (Appl. Microbiol. Biotechnol. 42: 901-909 (1995)) and Pseudomonas sp. 61-3 (Accession AB014757; Kato, et al. Appl. Microbiol. Biotechnol. 45: 363-370 (1996)).

A range of PHA synthase genes and genes encoding additional metabolic steps useful in PHA biosynthesis are described by Madison and Huisman. Microbiology and Molecular biology Reviews 63:21-53 (1999)) and Suriyamongkol et al. (Suriyamongkol et al., Biotechnol Adv, 2007, 25, 148-175).

Hydratase and Dehydrogenase

An alpha subunit of beta-oxidation multienzyme complex pertains to a multifunctional enzyme that minimally possesses hydratase and dehydrogenase activities. The subunit may also possess epimerase and A 3-cis, A 2-trans isomerase activities. Examples of alpha subunits of the beta-oxidation multienzyme complex are FadB from E. coli (DiRusso, C. C. J. Bacteriol. 1990, 172, 6459-6468), FaoA from Pseudomonas fragi (Sato, S., Hayashi, et al. J. Biochem. 1992, 111, 8-15), and the E. coli open reading frame 1714 that contains homology to multifunctional α subunits of the β-oxidation complex (Genbank Accession #1788682). A β subunit of the β-oxidation complex refers to a polypeptide capable of forming a multifunctional enzyme complex with its partner α subunit. The β subunit possesses thiolase activity. Examples of β subunits are FadA from E. coli (DiRusso, C. C. J. Bacteriol. 172: 6459-6468 (1990)), FaoB from Pseudomonas fragi (Sato, S., Hayashi, M., Imamura, S., Ozeki, Y., Kawaguchi, A. J. Biochem. 111: 8-15 (1992)), and the E. coli open reading frame f436 that contains homology to α subunits of the β-oxidation complex (Genbank Accession # AE000322; gene b2342).

Reductases

The transgene can encode a reductase. A reductase refers to an enzyme that can reduce β-ketoacyl CoAs to R-3-OH-acyl CoAs, such as the NADH dependent reductase from Chromatium vinosum (Liebergesell, M., & Steinbuchel, A. Eur. J. Biochem. 209: 135-150 (1992)), the NADPH dependent reductase from Alcaligenes eutrophus (Accession J04987, Peoples, O. P. & Sinskey, A. J. J. Biol. Chem. 264: 15293-15297 (1989))), the NADPH reductase from Zoogloea ramigera (Accession P23238; Peoples, O. P. & Sinskey, A. J. Molecular Microbiology 3: 349-357 (1989)) or the NADPH reductase from Bacillus megaterium (U.S. Pat. No. 6,835,820), Alcaligenes latus (Accession ALU47026), Rhizobium meliloti (Accession RMU17226), Paracoccus denitrificans (Accession D49362), Burkholderia sp. (Accession AF153086), Pseudomonas sp. strain 61-3 (Accession AB014757), Acinetobacter sp. strain RA3849 (Accession L37761), P. denitrificans, (Accession P50204), and Synechocystis sp. Strain PCC6803 (Taroncher-Oldenburg et al., (2000), Appl. Environ. Microbiol. 66: 4440-4448).

Thiolases

The transgene can encode a thiolase. A beta-ketothiolase refers to an enzyme that can catalyze the conversion of acetyl CoA and an acyl CoA to a β-ketoacyl CoA, a reaction that is reversible. An example of such thiolases are PhaA from Alcaligenes eutropus (Accession J04987, Peoples, O. P. & Sinskey, A. J. J. Biol. Chem. 264: 15293-15297 (1989)), BktB from Alcaligenes eutrophus (Slater et al. J Bacteriol. 180(8):1979-87 (1998)) and thiolases from the following Rhizobium meliloti (Accession RMU17226), Z. ramigera (Accession P07097), Paracoccus denitrificans (Accession D49362), Burkholderia sp. (Accession AF153086), Alcaligenes latus (Accession ALU47026), Allochromatium vinosum (Accession P45369), Thiocystis violacea (Accession P45363); Pseudomonas sp. strain 61-3 (Accession AB014757), Acinetobacter sp. strain RA3849 (Accession L37761) and Synechocystis sp. Strain PCC6803 (Taroncher-Oldenburg et al., (2000), Appl. Environ. Microbiol. 66: 4440-4448).

Oxidases

An acyl CoA oxidase refers to an enzyme capable of converting saturated acyl CoAs to Δ 2 unsaturated acyl CoAs. Examples of acyl CoA oxidases are POX1 from Saccharomyces cerevisiae (Dmochowska, et al. Gene, 1990, 88, 247-252) and ACX1 from Arabidopsis thaliana (Genbank Accession # AF057044).

Catalases

The transgene can also encode a catalase. A catalase refers to an enzyme capable of converting hydrogen peroxide to hydrogen and oxygen. Examples of catalases are KatB from Pseudomonas aeruginosa (Brown, et al.): Bacterial. 177: 6536-6544 (1995)) and KatG from E. coli (Triggs-Raine, B. L. & Loewen, P. C. Gene 52: 121-128 (1987)).

2. Promoters

Plant promoters can be selected to control the expression of the transgene in different plant tissues or organelles for all of which methods are known to those skilled in the art (Gasser & Fraley, Science 244:1293-99 (1989)). In one embodiment, promoters are selected from those of eukaryotic or synthetic origin that are known to yield high levels of expression in plant and algae cytosol. In another embodiment, promoters are selected from those of plant or prokaryotic origin that are known to yield high expression in plastids. In certain embodiments the promoters are inducible. Inducible plant promoters are known in the art.

Suitable constitutive promoters for nuclear-encoded expression include, for example, the core promoter of the Rsyn7 promoter and other constitutive promoters disclosed in U.S. Pat. No. 6,072,050; the core CAMV 355 promoter, (Odell et al. (1985) Nature 313:810-812); rice actin (McElroy et al. (1990) Plant Cell 2:163-171); ubiquitin (Christensen et al. (1989) Plant Mol. Biol. 12:619-632 and Christensen et al. (1992) Plant Mol. Biol. 18:675-689); pEMU (Last et al. (1991) Theor. Appl. Genet. 81:581-588); MAS (Velten et al. (1984) EMBO J. 3:2723-2730); and ALS promoter (U.S. Pat. No. 5,659,026). Other constitutive promoters include, for example, U.S. Pat. Nos. 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785; 5,399,680; 5,268,463; 5,608,142.

“Tissue-preferred” promoters can be used to target a gene expression within a particular tissue such as seed, leaf or root tissue. Tissue-preferred promoters include Yamamoto et al. (1997) Plant J 12(2)255-265; Kawamata et al. (1997) Plant Cell Physiol. 38(7):792-803; Hansen et al (1997) Mol. Gen. Genet. 254(3):337-343; Russell et al. (1997) Transgenic Res. 6(2):157-168; Rinehart et al. (1996) Plant Physiol. 112(3):1331-1341; Van Camp et al (1996) Plant Physiol. 112(2):525-535; Canevascini et al. (1996) Plant Physiol. 112(2):513-524; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778; Lam (1994) Results Probl. Cell Differ. 20:181-196; Orozco et al. (1993) Plant Mol. Biol. 23(6):1129-1138; Matsuoka et al. (1993) Proc Natl. Acad. Sci. USA 90(20):9586-9590; and Guevara-Garcia et al. (1993) Plant J. 4(3):495-505.

“Seed-preferred” promoters include both “seed-specific” promoters (those promoters active during seed development such as promoters of seed storage proteins) as well as “seed-germinating” promoters (those promoters active during seed germination). See Thompson et al. (1989) BioEssays 10:108. Such seed-preferred promoters include, but are not limited to, Cim1 (cytokinin-induced message); cZ19B1 (maize 19 kDa zein); milps (myo-inositol-1-phosphate synthase); and ce1A (cellulose synthase). Gama-zein is a preferred endosperm-specific promoter. Glob-1 is a preferred embryo-specific promoter. For dicots, seed-specific promoters include, but are not limited to, bean β-phaseolin, napin β-conglycinin, soybean lectin, cruciferin, oleosin, the Lesquerella hydroxylase promoter, and the like. For monocots, seed-specific promoters include, but are not limited to, maize 15 kDa zein, 22 kDa zein, 27 kDa zein, g-zein, waxy, shrunken 1, shrunken 2, globulin 1, etc. Additional seed specific promoters useful for practicing this invention are described in the Examples disclosed herein.

Leaf-specific promoters are known in the art. See, for example, Yamamoto et al. (1997) Plant J. 12(2):255-265; Kwon et al. (1994) Plant Physiol. 105:357-67; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778; Gotor et al. (1993) Plant J. 3:509-18; Orozco et al. (1993) Plant Mol. Biol. 23(6):1129-1138; and Matsuoka et al. (1993) Proc. Natl. Acad. Sci. USA 90(20):9586-9590.

Root-preferred promoters are known and may be selected from the many available from the literature or isolated de nova from various compatible species. See, for example, Hire et al. (1992) Plant Mol. Biol. 20(2): 207-218 (soybean root-specific glutamine synthetase gene); Keller and Baumgartner (1991) Plant Cell 3(10):1051-1061 (root-specific control element in the GRP 1.8 gene of French bean); Sanger et al. (1990) Plant Mol. Biol. 14(3):433-443 (root-specific promoter of the mannopine synthase (MAS) gene of Agrobacterium tumefaciens); and Miao et al. (1991) Plant Cell 3(1):1 1′-22 (full-length cDNA clone encoding cytosolic glutamine synthetase (GS), which is expressed in roots and root nodules of soybean). See also U.S. Pat. Nos. 5,837,876; 5,750,386; 5,633,363; 5,459,252; 5,401,836; 5,110,732; and 5,023,179.

Plastid specific promoters include the PrbcL promoter [Allison L. A. et al., EMBO 15: 2802-2809 (1996); Shiina T. et al., Plant Cell 10: 1713-1722 (1998)]; the PpsbA promoter [Agrawal G K, et al., Nucleic Acids Research 29: 1835-1843 (2001)]; the Prrn 16 promoter [Svab Z & Maliga P., Proc. Natl. Acad. Sci. USA 90: 913-917 (1993), Allison L A et al., EMBO 15: 2802-2809 (1996)]; the PaccD promoter (WO97/06250; Hajdukiewicz P T J et al., EMBO J. 16: 4041-4048 (1997)).

Chemical-regulated promoters can be used to modulate the expression of a gene in a plant through the application of an exogenous chemical regulator. Depending upon the objective, the promoter may be a chemical-inducible promoter, where application of the chemical induces gene expression, or a chemical-repressible promoter, where application of the chemical represses gene expression. Chemical-inducible promoters are known in the art and include, but are not limited to, the maize 1n2-2 promoter, which is activated by benzenesulfonamide herbicide safeners, the maize GST promoter, which is activated by hydrophobic electrophilic compounds that are used as pre-emergent herbicides, and the tobacco PR-1 a promoter, which is activated by salicylic acid. Other chemical-regulated promoters of interest include steroid-responsive promoters (see, for example, the glucocorticoid-inducible promoter in Schena et al. Proc. Natl. Acad. Sci. USA 88:10421-10425 (1991) and McNellis et al. Plant J 14(2):247-257 (1998)) and tetracycline-inducible and tetracycline-repressible promoters (see, for example, Gatz et al. Mol. Gen. Genet. 227:229-237 (1991), and U.S. Pat. Nos. 5,814,618 and 5,789,156), herein incorporated by reference in their entirety.

In one embodiment, coordinated expression of the three transgenes, phaA, phaB, and phaC, necessary for conversion of acetyl-CoA to PHB is controlled by a seed specific promoter, such as the soybean oleosin promoter (Rowley et al., Biochim Biophys Acta, 1997, 1345, 1-4) or the promoter from the lesquerlla hydroxylase gene (U.S. Pat. No. 6,437,220 B1). In another embodiment, coordinated expression of the three transgenes, phaA, phaB, and phaC, necessary for conversion of acetyl-CoA to PHB is controlled by a promoter active primarily in the biomass plant, such as the maize chlorophyll A/B binding protein promoter (Sullivan et al., Mol. Gen. Genet., 1989, 215, 431-40). It has been previously shown that plants transformed with multi-gene constructs produced higher levels of polymer than plants obtained from crossing single transgene lines (Valentin et al., Int. J. Biol. Macromol., 1999, 25, 303-306; Bohmert et al., Planta, 2000, 211, 841-845).

In one embodiment, the final molecular weight of the polymer produced is controlled by the choice of promoter for expression of the PHA synthase gene. As described in U.S. Pat. No. 5,811,272, high PHA synthase activity will lower polymer molecular weight and low PHA synthase activity will increase polymer molecular weight. In another embodiment, a strong promoter is used for expression of the genes encoding plastid-targeted monomer producing enzymes while a weaker promoter is used to control expression of synthase.

3. Transcription Termination Sequences

At the extreme 3′ end of the transcript of the transgene, a polyadenylation signal can be engineered. A polyadenylation signal refers to any sequence that can result in polyadenylation of the mRNA in the nucleus prior to export of the mRNA to the cytosol, such as the 3′ region of nopaline synthase (Bevan, M., Barnes, W. M., Chilton, M. D. Nucleic Acids Res. 1983, 11, 369-385).

4. Selectable Markers

Genetic constructs may encode a selectable marker to enable selection of plastid transformation events. There are many methods that have been described for the selection of transformed plants [for review see (Miki et al., Journal of Biotechnology, 2004, 107, 193-232) and references incorporated within]. Selectable marker genes that have been used extensively in plants include the neomycin phosphotransferase gene nptII (U.S. Pat. No. 5,034,322, U.S. Pat. No. 5,530,196), hygromycin resistance gene (U.S. Pat. No. 5,668,298), the bar gene encoding resistance to phosphinothricin (U.S. Pat. No. 5,276,268), the expression of aminoglycoside 3″-adenyltransferase (aadA) to confer spectinomycin resistance (U.S. Pat. No. 5,073,675), the use of inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase (U.S. Pat. No. 4,535,060) and methods for producing glyphosate tolerant plants (U.S. Pat. No. 5,463,175; U.S. Pat. No. 7,045,684). Methods of plant selection that do not use antibiotics or herbicides as a selective agent have been previously described and include expression of glucosamine-6-phosphate deaminase to inactive glucosamine in plant selection medium (U.S. Pat. No. 6,444,878) and a positive/negative system that utilizes D-amino acids (Erikson et al., Nat Biotechnol, 2004, 22, 455-8). European Patent Publication No. EP 0 530 129 A1 describes a positive selection system which enables the transformed plants to outgrow the non-transformed lines by expressing a transgene encoding an enzyme that activates an inactive compound added to the growth media. U.S. Pat. No. 5,767,378 describes the use of mannose or xylose for the positive selection of transgenic plants. Methods for positive selection using sorbitol dehydrogenase to convert sorbitol to fructose for plant growth have also been described (WO 2010/102293). Screenable marker genes include the beta-glucuronidase gene (Jefferson et al., 1987, EMBO J. 6: 3901-3907; U.S. Pat. No. 5,268,463) and native or modified green fluorescent protein gene (Cubitt et al., 1995, Trends Biochem. Sci. 20: 448-455; Pan et al., 1996, Plant Physiol. 112: 893-900).

Transformation events can also be selected through visualization of fluorescent proteins such as the fluorescent proteins from the nonbioluminescent Anthozoa species which include DsRed, a red fluorescent protein from the Discosoma genus of coral (Matz et al. (1999), Nat Biotechnol 17: 969-73). An improved version of the DsRed protein has been developed (Bevis and Glick (2002), Nat Biotech 20: 83-87) for reducing aggregation of the protein. Visual selection can also be performed with the yellow fluorescent proteins (YFP) including the variant with accelerated maturation of the signal (Nagai, T. et al. (2002), Nat Biotech 20: 87-90), the blue fluorescent protein, the cyan fluorescent protein, and the green fluorescent protein (Sheen et al. (1995), Plant J 8: 777-84; Davis and Vierstra (1998), Plant Molecular Biology 36: 521-528). A summary of fluorescent proteins can be found in Tzfira et al. (Tzfira et al. (2005), Plant Molecular Biology 57: 503-516) and Verkhusha and Lukyanov (Verkhusha, V. V. and K. A. Lukyanov (2004), Nat Biotech 22: 289-296) whose references are incorporated in entirety. Improved versions of many of the fluorescent proteins have been made for various applications. Use of the improved versions of these proteins or the use of combinations of these proteins for selection of transformants will be obvious to those skilled in the art. It is also practical to simply analyze progeny from transformation events for the presence of the PHB thereby avoiding the use of any selectable marker.

For plastid transformation constructs, a preferred selectable marker is the spectinomycin-resistant allele of the plastid 16S ribosomal RNA gene (Staub J M, Maliga P, Plant Cell 4: 39-45 (1992); Svab Z, Hajdukiewicz P, Maliga P, Proc. Natl., Acad. Sci. USA 87: 8526-8530 (1990)). Selectable markers that have since been successfully used in plastid transformation include the bacterial aadA gene that encodes aminoglycoside adenyltransferase (AadA) conferring spectinomycin and streptomycin resistance (Svab et al., Proc, Natl. Acad. Sci. USA, 1993, 90, 913-917), nptII that encodes aminoglycoside phosphotransferase for selection on kanamycin (Carrer H, Hockenberry T N, Svab Z, Maliga P., Mol. Gen. Genet. 241: 49-56 (1993); Lutz K A, et al., Plant J. 37: 906-913 (2004); Lutz K A, et al., Plant Physiol. 145: 1201-1210 (2007)), aphA6, another aminoglycoside phosphotransferase (Huang F—C, et al, Mol. Genet. Genomics 268: 19-27 (2002)), and chloramphenicol acetyltransferase (Li, W., et al. (2010), Plant Mol Biol, DOI 10.1007/s11103-010-9678-4). Another selection scheme has been reported that uses a chimeric betaine aldehyde dehydrogenase gene (BADH) capable of converting toxic betaine aldehyde to nontoxic glycine betaine (Daniell H, et al., Curr. Genet. 39: 109-116 (2001)).

5. Plastid Targeting Signals

Plastid targeting sequences are known in the art and include the chloroplast small subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) (de Castro Silva Filho et al, Plant Mal. Biol. 30:769-780 (1996); Schnell et J. Biol. Chem. 266(5):3335-3342 (1991)); 5-(enolpyruvyl)shikimate-3-phosphate synthase (EPSPS) (Archer et al. J. Bioenerg. Biomemb. 22(6):789-810 (1990)); tryptophan synthase (Zhao et al. J. Biol. Chem. 270(11):6081-6087 (1995)); plastocyanin (Lawrence et al. J. Biol. Chem. 272(33):20357-20363 (1997)); chorismate synthase (Schmidt et al. J. Biol. Chem. 268(36):27447-27457 (1993)); and the light harvesting chlorophyll a/b binding protein (LHBP) (Lamppa et al. J. Biol. Chem. 263:14996-14999 (1988)). See also Von Heijne et al. Plant Mol. Biol. Rep. 9:104-126 (1991); Clark et al. J. Biol. Chem. 264:17544-17550 (1989); Della-Cioppa et al. Plant Physiol. 84:965-968 (1987); Romer et al. Biochem. Biophys. Res. Commun. 196:1414-1421 (1993); and Shah et al. Science 233:478-481 (1986). Alternative plastid targeting signals have also been described in the following: US 2008/0263728; Miras, S. et al. (2002), J Biol Chem 277(49): 47770-8; Miras, S. et al. (2007), J Biol Chem 282: 29482-29492.

B. Exemplary Host Plants

Plants transformed in accordance with the present disclosure may be monocots or dicots. The transformation of suitable agronomic plant hosts using vectors for nuclear transformation or direct plastid transformation can be accomplished with a variety of methods and plant tissues. Representative plants useful in the methods disclosed herein include the Brassica family including B. napus, B. rapa, B. carinata and B. juncea; industrial oilseeds such as Camelina sativa, Crambe, jatropha, castor; Calendula, Cuphea, Arabidopsis thaliana; maize; soybean; cottonseed; sunflower; palm; coconut; safflower; peanut; mustards including Sinapis alba; sugarcane flax and tobacco, also are useful with the methods disclosed herein. Representative tissues for transformation using these vectors include protoplasts, cells, callus tissue, leaf discs, pollen, and meristems.

C. Methods of Plant Transformation

Transformation protocols as well as protocols for introducing nucleotide sequences into plants may vary depending on the type of plant or plant cell targeted for transformation. Suitable methods of introducing nucleotide sequences into plant cells and subsequent insertion into the plant genome include microinjection (Crossway et al. (1986) Biotechniques 4:320-334), electroporation (Riggs et al. (1986) Proc. Natl. Acad. Sci. USA 83:5602-5606), Agrobacterium-mediated transformation (Townsend et al., U.S. Pat. No. 5,563,055; Zhao et al. WO US98/01268), direct gene transfer (Paszkowski et al. (1984) EMBO J. 3:2717-2722), and ballistic particle acceleration (see, for example, Sanford et al., U.S. Pat. No. 4,945,050; Tomes et al. (1995) Plant Cell, Tissue, and Organ Culture: Fundamental Methods, ed. Gamborg and Phillips (Springer-Verlag, Berlin); and McCabe et al. Biotechnology 6:923-926 (1988)). Also see Weissinger et al. Ann. Rev. Genet. 22:421-477 (1988); Sanford et al, Particulate Science and Technology 5:27-37 (1987) (onion); Christou et al. Plant Physiol. 87:671-674 (1988) (soybean); McCabe et al. (1988) BioTechnology 6:923-926 (soybean); Finer and McMullen In Vitro Cell Dev. Biol. 27P:175-182 (1991) (soybean); Singh et al. Theor. Appl. Genet. 96:319-324 (1998) (soybean); Dafta et al. (1990) Biotechnology 8:736-740 (rice); Klein et al. Proc. Natl. Acad. Sci. USA 85:4305-4309 (1988) (maize); Klein et al. Biotechnology 6:559-563 (1988) (maize); Tomes, U.S. Pat. No. 5,240,855; Buising et al., U.S. Pat. Nos. 5,322,783 and 5,324,646; Tomes et al. (1995) in Plant Cell, Tissue, and Organ Culture Fundamental Methods, ed. Gamborg (Springer-Verlag, Berlin) (maize); Klein et al. Plant Physiol. 91:440-444 (1988) (maize); Fromm et al. Biotechnology 8:833-839 (1990) (maize); Hooykaas-Van Slogteren et al. Nature 311:763-764 (1984); Bowen et al., U.S. Pat. No. 5,736,369 (cereals); Bytebier et al. Proc. Natl. Acad. Sci. USA 84:5345-5349 (1987) (Liliaceae); De Wet et al. in The Experimental Manipulation of Ovule Tissues, ed. Chapman et al. (Longman, N.Y.), pp. 197-209 (1985) (pollen); Kaeppler et al. Plant Cell Reports 9:415-418 (1990) and Kaeppler et al. Theor. Appl. Genet. 84:560-566 (1992) (whisker-mediated transformation); D'Halluin et al. Plant Cell 4:1495-1505 (1992) (electroporation); Li et al. Plant Cell Reports 12:250-255 (1993) and Christou and Ford Annals of Botany 75:407-413 (1995) (rice); Osjoda et al. Nature Biotechnology 14:745-750 (1996) (maize via Agrobacterium tumefaciens); all of which are herein incorporated by reference in their entirety. References for protoplast transformation and/or gene gun for Agrisoma technology are described in WO 2010/037209. Methods for transforming plant protoplasts are available including transformation using polyethylene glycol (PEG), electroporation, and calcium phosphate precipitation (see for example Potrykus et al., 1985, Mol. Gen. Genet., 199, 183-188; Potrykus et al., 1985, Plant Molecular Biology Reporter, 3, 117-128), Methods for plant regeneration from protoplasts have also been described [Evans et al., in Handbook of Plant Cell Culture, Vol 1, (Macmillan Publishing Co., New York, 1983); Vasil, 1K in Cell Culture and Somatic Cell Genetics (Academic, Orlando, 1984)].

Methods for transformation of plastids such as chloroplasts are known in the art. See, for example, Svab et al. (1990) Proc. Natl. Acad. Sci. USA 87:8526-8530; Svab and Maliga (1993) Proc. Natl. Acad. Sci. USA 90:913-917; Svab and Maliga (1993) EMBO J. 12:601-606. The method relies on particle gun delivery of DNA containing a selectable marker and targeting of the DNA to the plastid genome through homologous recombination. Additionally, plastid transformation may be accomplished by transactivation of a silent plastid-borne transgene by tissue-preferred expression of a nuclear-encoded and plastid-directed RNA polymerase (McBride et al., Proc. Natl. Acad. Sci. USA, 1994, 91:7301-7305) or by use of an integrase, such as the phiC31 phage site-specific integrase, to target the gene insertion to a previously inserted phage attachment site (Lutz et al., Plant J, 2004, 37, 906-13). Plastid transformation vectors can be designed such that the transgenes are expressed from a promoter sequence that has been inserted with the transgene during the plastid transformation process or, alternatively, from an endogenous plastidial promoter such that an extension of an existing plastidial operon is achieved (Herz et al., Transgenic Research, 2005, 14, 969-982). An alternative method for plastid transformation as described in WO 2010/061186 wherein RNA produced in the nucleus of a plant cell can be targeted to the plastid genome can also be used to practice the disclosed invention. Inducible gene expression from the plastid genome using a synthetic riboswitch has also been reported (Verhounig et al. (2010), Proc Natl Acad Sci USA 107: 6204-6209). Methods for designing plastid transformation vectors are described by Lutz et al. (Lutz et al., Plant Physiol, 2007, 145, 1201-10).

Recombinase technologies which are useful for producing the disclosed transgenic plants include the cre-lox, FLP/FRT and Gin systems. Methods by which these technologies can be used for the purpose described herein are described for example in (U.S. Pat. No. 5,527,695; Dale And Ow, 1991, Proc. Natl. Acad. Sci. USA 88: 10558-10562; Medberry et al., 1995, Nucleic Acids Res. 23: 485-490).

D. Methods for Reproducing Transgenic Plants

Following transformation by any one of the methods described above, the following procedures can be used to obtain a transformed plant expressing the transgenes: select the plant cells that have been transformed on a selective medium; regenerate the plant cells that have been transformed to produce differentiated plants; select transformed plants expressing the transgene producing the desired level of desired polypeptide(s) in the desired tissue and cellular location.

In plastid transformation procedures, further rounds of regeneration of plants from explants of a transformed plant or tissue can be performed to increase the number of transgenic plastids such that the transformed plant reaches a state of homoplasmy (all plastids contain uniform plastomes containing transgene insert).

The cells that have been transformed may be grown into plants in accordance with conventional techniques. See, for example, McCormick et al, Plant Cell Reports 5:81-84 (1986). These plants may then be grown, and either pollinated with the same transformed variety or different varieties, and the resulting hybrid having constitutive expression of the desired phenotypic characteristic identified. Two or more generations may be grown to ensure that constitutive expression of the desired phenotypic characteristic is stably maintained and inherited and then seeds harvested to ensure constitutive expression of the desired phenotypic characteristic has been achieved.

In some scenarios, it may be advantageous to insert a multi-gene pathway into the plant by crossing of lines containing portions of the pathway to produce hybrid plants in which the entire pathway has been reconstructed. This is especially the case when high levels of product in a seed compromises the ability of the seed to germinate or the resulting seedling to survive under normal soil growth conditions. Hybrid lines can be created by crossing a line containing one or more PHB genes with a line containing the other gene(s) needed to complete the PHB biosynthetic pathway. Use of lines that possess cytoplasmic male sterility (Esser, K. et al., 2006, Progress in Botany, Springer Berlin Heidelberg. 67, 31-52) with the appropriate maintainer and restorer lines allows these hybrid lines to be produced efficiently. Cytoplasmic male sterility systems are already available for some Brassicaceae species (Esser, K. et al., 2006, Progress in Botany, Springer Berlin Heidelberg. 67, 31-52). These Brassicaceae species can be used as gene sources to produce cytoplasmic male sterility systems for other oilseeds of interest such as Camelina.

III. Methods for Use

The disclosed genetic constructs can be used to produce industrial oilseed plants for high levels of PHA production. Specifically, PHA is produced in the seed.

The transgenic plants can be grown and harvested. The polyhydroxyalkanoate can be isolated from the oilseeds and the remaining plant material can be used as a feedstock for industrial use, preferably for the production of oleochemicals, energy or for use as feed for animals. The polyhydroxyalkanoate harvested from the plants can then be used to produce plastics, rubber material, coating material, and binders for paints, or as a feedstock for producing chemical derivatives such as hydroxyacids, esters, alkenoic acids or amines. PHA also has several medical applications.

The present invention will be further understood by reference to the following non-limiting examples.

EXAMPLES

Example 1

Design and Construction of Transformation Vectors for Production of PHB in Oilseeds

Five different vectors for seed specific expression of the PHB pathway were constructed containing different seed specific promoters for production of PHB in oilseeds (Table 1). Vector pMBXS490, a pCAMBIA based plasmid (Centre for Application of Molecular Biology to International Agriculture, Canberra, Australia), contains the following gene expression cassettes: (1) an expression cassette for PHA synthase containing the promoter from the soybean oleosin isoform A gene, a DNA fragment encoding the signal peptide of the small subunit of rubisco from pea (P. sativum) and the first 24 amino acids of the mature protein (Cashmore, A. R. 1983, In Genetic Engineering of Plants, pp. 29-38), a DNA fragment encoding a hybrid PHA synthase (PhaC; U.S. Pat. No. 6,316,262) in which the first nine amino acids at the N-terminus of this synthase are derived from the Pseudomonas oleovorans phaC1 gene and the remainder of the synthase coding sequence is derived from Zoogloea ramigera phaC gene, and the 3′ termination sequence from the soybean oleosin isoform A gene; (2) an expression cassette for reductase containing the promoter from the soybean oleosin isoform A gene, a DNA fragment encoding the signal peptide and the first 24 amino acids of the mature protein of the small subunit of rubisco from pea, a DNA fragment encoding a NADPH dependent reductase (PhaB) from Ralstonia eutropha eutropha (Peoples, O. & A. Sinskey, 1989, J. Biol. Chem., 264, 15293-15297), and the 3′ termination sequence from the soybean oleosin isoform A gene; (3) an expression cassette for thiolase containing the promoter from the soybean glycinin (gy1) gene (Iida et al., 1995, Plant Cell Reports, 14, 539-544), a DNA fragment encoding the signal peptide and the first 24 amino acids of the mature protein of the small subunit of rubisco from pea, the phaA gene encoding a β-ketothiolase (PhaA) from Ralstonia eutropha (Peoples, O. & A. Sinskey, 1989, J. Biol. Chem., 264, 15293-15297), and a 3′ termination sequence from the soybean glycinin gene; (4) an expression cassette for DsRed, a protein that can be visualized in seeds by placing them in light of the appropriate wavelength, containing the promoter from the cassava mosaic virus (CMV), a DNA fragment encoding a modified red fluorescent protein from Discosoma sp. (DsRed) in which eleven amino acids have been added to the C-terminus to increase solubility and/or prevent aggregation of the protein, and a termination sequence from the Agrobacterium tumefaciens nopaline synthase gene.

TABLE 1
Summary of transformation vectors
containing seed specific promoters

		Promoter controlling	Selectable or
	Plasmid	expression of pha genes	visible marker
	pMBXS490	Oleosin	DsRed
	pMBXS364	LH	DsRed
	pMBXS355	LH	bar
	pMBXS491	Napin	DsRed
	pMBXS492	Glycinin	DsRed

Promoters are as follows: LH, promoter from the Lesquerella fendleri bifunctional oleate 12-hydroxylase:saturate gene (U.S. Pat. No. 6,437,220 B1); Oleosin, promoter from the soybean oleosin isoform A gene (Rowley and Herman, 1997, Biochim. Biophys. Acta 1345, 1-4); Napin, promoter from the Brassica napes napin gene (Ellenstrom, M. et al., 1996, Plant Molecular Biology, 32: 1019-1027); Glycinin, promoter from the soybean glycinin (gy1) gene (Iida, A. et al., 1995, Plant Cell Reports, 14:539-544).

Vectors pMBXS364, pMBXS355, pMBXS491, and pMBXS492 contain the same PHB pathway genes as pMBXS490 with the exception that the expression of these genes is under the control of different promoters as outlined in Table 1. Vector pMBXS355 contains an expression cassette for the bar gene, encoding phosphinothricin acetyltransferase whose expression is under the control of the 35S promoter. Expression of the bar gene allows selection of transformants based on their resistance to bialaphos. All other vectors in Table 1 contain expression cassettes for DsRed allowing the identification of transgenic seeds under the appropriate wavelength of light.

Example 2

Transformation of Camelina

In preparation for plant transformation experiments, seeds of Camelina sativa cultivar Suneson or Celine were sown directly into 4 inch pots filled with soil (Metro mix) in the greenhouse. Growth conditions were maintained at 24° C. during the day and 18° C. during the night. Plants were grown until flowering. Plants with a number of unopened flower buds were used in ‘floral dip’ transformations.

Agrobacterium strain GV3101 was transformed with the construct of interest using electroporation. A single colony of GV3101 containing the construct of interest was obtained from a freshly streaked plate and was inoculated into 5 mL LB medium. After overnight growth at 28° C., 2 mL of culture was transferred to a 500-mL flask containing 300 mL of LB and incubated overnight at 28° C. Cells were pelleted by centrifugation (6,000 rpm, 20 min), and diluted to an OD600 of ˜0.8 with infiltration medium containing 5% sucrose and 0.05% (v/v) Silwet-L77 (Lehle Seeds, Round Rock, Tex., USA). Camelina plants were transformed by “floral dip” using transformation constructs as follows. Pots containing plants at the flowering stage were placed inside a 460 mm height vacuum desiccator (Bel-Art, Pequannock, N.J., USA). Inflorescences were immersed into the Agrobacterium inoculum contained in a 500-ml beaker. A vacuum (85 kPa) was applied and held for 5 min. Plants were removed from the desiccator and were covered with plastic bags in the dark for 24 h at room temperature. Plants were removed from the bags and returned to normal growth conditions within the greenhouse for seed formation.

To identify Camelina seeds expressing DsRed, fully mature seeds were harvested from transformed plants and placed in a desiccator with anhydrous calcium sulfate as desiccant for at least 2 days prior to screening. DsRed expressing seeds were visualized in a darkroom with a green LumaMax LED flashlight (Lab Safety Supply, Inc., Janesville, Wis.) and a pair of KD's Dark Red glasses (Pacific Coast Sunglasses Inc., Santa Maria, Calif.).

To identify bialaphos resistant seeds, seeds from floral dip transformations were sterilized in 70% ethanol and 10% bleach, and washed in water. Sterilized seeds were placed on germination and selection medium in square Petri dishes. The germination and selection medium contained 10 mg/L bialaphos (Gold BioTechnology, 130178-500) in ½× MS medium, which was made with Murashige & Skoog medium mixture (Caisson Labs, MSP09) at half concentration. The plates were sealed and placed in a growth chamber for germination under a 16-h photoperiod, 3,000 lux light intensity, and temperatures of 23/20° C. at day/night. Seedlings with greenish cotyledons were picked and transferred to soil about six days after initiation of germination.

Example 3

Production of PHB in Seeds of Camelina

In initial transformation experiments with pMBXS490, 24 DsRed positive seeds were isolated. Four of these seeds were sacrificed to determine their PHB content using a previously described gas chromatography/butanolysis technique performed essentially as previously described (Somleva et al., 2008, Plant Biotechnol. J., 663-678). These four seeds contained 19.9, 12.0, 9.8, and 6.4% dwt PHB in the seed. When other seeds from this transformation were planted in soil, seedlings possessed whitish cotyledons and their growth was severely impaired. Only a few T₁ seeds with low levels of PHB were capable of germination and survival in soil in a greenhouse. These seedlings were still weak and possessed white or variegated cotyledons.

In transformations of pMBXS355 and pMBXS364, seeds from transformed plants were screened for resistance to bialophos and or visual screening for DsRed, respectively. Despite having the same promoter controlling the expression of the PHB biosynthetic pathway, the maximum PHB production in pMBXS355 (0.54% PHB) was significantly lower than the amount produced by pMBXS364 (3.4%) (Table 2). This is likely due to difficulty in distinguishing between weak pMBXS355 seedlings that produced higher levels of PHB and the non-transformed, bialophos sensitive seedlings.

TABLE 2
Comparison of PHB production in Lines isolated
using bialaphos selection or visual screening

	Selectable or	# of	# of Lines w/	Range of PHB
	Screenable	Lines	PHB in T2	Production
Vector	Marker	Tested	Seeds	(% seed weight)

pMBXS355	Bar1	204	5	0.05 to 0.54%
pMBXS364	DsRed2	170	85	0.5 to 3.4%
1Selection of transformants performed by germination of seeds on tissue culture plates containing 10 mg/L bialophos.
2Selection of transformants performed by visual screening for DsRed expression.

In transformations with pMBX491 and pMBX492 containing the PHB genes under the control of the napin and glycinin promoters, respectively, were healthier than transformants obtained from pMBX490 transformations. For pMBX491, T2 seeds were isolated containing 8% PHB in DsRed seeds picked from the segregating population. These seeds possessed a 75% germination rate and a 60% survival rate under greenhouse conditions in soil. The cotyledons after 11 days were chlorotic and the growth of this line was significantly delayed compared to wild-type. For pMBX492, T2 seeds were isolated containing 6.9% PHB in DsRed seeds picked from the segregating population. These seeds possessed a 75% germination rate and a 70% survival rate under greenhouse conditions in soil. After 11 days, the cotyledons and first true leaves of this transformant were green. The growth of this line was somewhat delayed compared to wild-type but faster than the pMBXS491 line.

The 19% dwt PHB produced in a single seed obtained from Camelina plants transformed with construct pMBXS490 was an unexpected result and is the highest level of PHB reported in oilseeds to date. Previous studies with Brassica napus produced up to 7.7% dwt PHB. These seeds were obtained from transformation of Brassica napus using stem segments as the explants and selection of the transformed explants (Fry, J. et al., 1987, 6, 321-325) using glyphosate resistance obtained from expression of a gene encoding 5-enolpyruvylshikimate-3-phosphate synthase. Researchers did not report any germination issues with seeds isolated from the transformed plants [Houmiel et al., 1999, Planta, 209, 547-550; Valentin et al., 1999, Int. J. Biol. Macromol. 25, 303-306].

The use of DsRed as a visual marker in Camelina enabled the identification of high PHB producing seeds that would not have germinated in a typical seed screening procedure where an antibiotic or herbicide selectable marker, such as glyphosate resistance, is employed to provide resistance to the selection agent during seed germination and seedling development in tissue culture medium.

Example 4

Transformation of Brassica napus, Brassica carinata, and Brassica juncea

Transformation of Brassica carinata

Brassica carinata can be transformed using a previously described floral dip method (Shiv et al., 2008, Journal of Plant Biochemistry and Biotechnology 17, 1-4). Briefly constructs of interest are transformed into Agrobacterium strain GV-3101 and cells are grown in liquid medium. Cells are harvested and resuspended in a transformation medium consisting of ½ MS salts, 5% sucrose, and 0.05% Silwet L-77. Brassica carinata plants are grown in a greenhouse until inflorescences develop and approximately 25% of their flowers are opened. Plants are submerged in the prepared Agrobacterium solution for approximately 1 minute, and covered for 24 hours. Plants are returned to the greenhouse and allowed to set seed. Transformed seeds are screened by picking DsRed seeds under the appropriate wavelength of light as described above.

Transformation of Brassica napus

Brassica seeds are surface sterilized in 10% commercial bleach (Javex, Colgate-Palmolive) for 30 min with gentle shaking. The seeds are washed three times in sterile distilled water and placed in germination medium comprising Murashige-Skoog (MS) salts and vitamins, 3% (w/v) sucrose and 0.7% (w/v) phytagar, pH 5.8 at a density of 20 per plate and maintained at 24° C. an a 16 h light/8 h dark photoperiod at a light intensity of 60-80 μEm⁻² s⁻¹ for 4-5 days.

Constructs of interest are introduced into Agrobacterium tumefacians strain EHA101 (Hood et. al., 1986, J. Bacteriol. 168: 1291-1301) by electroporation. Prior to transformation of cotyledonary petioles, single colonies of strain EHA101 harboring each construct are grown in 5 ml of minimal medium supplemented with appropriate antibiotics for 48 hr at 28° C. One ml of bacterial suspension was pelleted by centrifugation for 1 min in a microfuge. The pellet was resuspended in 1 ml minimal medium.

For transformation, cotyledons are excised from 4 or in some cases 5 day old seedlings so that they included ˜2 mm of petiole at the base. Individual cotyledons with the cut surface of their petioles are immersed in diluted bacterial suspension for 1 s and immediately embedded to a depth of ˜2 mm in co-cultivation medium, MS medium with 3% (w/v) sucrose and 0.7% phytagar and enriched with 20 μM benzyladenine. The inoculated cotyledons are plated at a density of 10 per plate and incubated under the same growth conditions for 48 h. After co-cultivation, the cotyledons are transferred to regeneration medium comprising MS medium supplemented with 3% sucrose, 20 μM benzyladenine, 0.7% (w/v) phytagar, pH 5.8, 300 mg/L timentinin and 20 mg/L kanamycin sulfate.

After 2-3 weeks regenerant shoots obtained are cut and maintained on “shoot elongation” medium (MS medium containing, 3% sucrose, 300 mg/L timentin, 0.7% (w/v) phytagar, 300 mg/L timentinin and 20 mg/L kanamycin sulfate, pH 5.8) in Magenta jars. The elongated shoots are transferred to “rooting” medium comprising MS medium, 3% sucrose, 2 mg/L indole butyric acid, 0.7% phytagar and 500 mg/L carbenicillin. After roots emerge, plantlets are transferred to potting mix (Redi Earth, W.R. Grace and Co.). The plants are maintained in a misting chamber (75% relative humidity) under the same growth conditions. Plants are allowed to self pollinate to produce seeds. Seeds are screened by visualization of DsRed as described above.

Brassica napus can also be transformed using the floral dip procedure described by Shiv et al. (Shiv et al., 2008, Journal of Plant Biochemistry and Biotechnology 17, 1-4) as described above for Brassica carinata.

Transformation of Brassica juncea

Brassica juncea can be transformed using hypocotyl explants according to the methods described by Barfield and Pua (Barfield and Pua, Plant Cell Reports, 10, 308-314) or Pandian et al. (Pandian, et al., 2006, Plant Molecular Biology Reporter 24: 103a-103i) as follows.

B. juncea seeds are sterilized 2 min in 70% (v/v) ethanol and washed for 20 min in 25% commercial bleach (10 g/L hypochlorite). Seeds are rinsed 3× in sterile water. Surface-sterilized seeds are plated on germination medium (1× MS salts, 1×MS vitamins, 30 g/L sucrose, 500 mg/L MES. pH 5.5) and kept in the cold room for 2 days. Seeds are incubated for 4-6 days at 24° C. under low light (20 μm m⁻¹s⁻¹). Hypocotyl segments are excised and rinsed in 50 mL of callus induction medium (1× MS salts, 1× B5 vitamins, 30 g/L sucrose, 500 mg/L MES, 1.0 mg/L 2.4-D, 1.0 mg/L kinetin pH 5.8) for 30 min without agitation. This procedure is repeated but with agitation on orbital shaker (˜140 g) for 48 h at 24° C. in low light (10 μm m⁻¹s⁻¹).

Agrobacterium can be prepared as follows: Cells of Agrobacterium strain AGL1 (Lazo, G. et al. (1991), Biotechnology, 9: 963-967) containing the construct of interest are grown in 5 mL of LB medium with appropriate antibiotic at 28° C. for 2 days. The 5 mL culture is transferred to 250 mL flask with 45 mL of LB and cultured for 4 h at 28° C. Cells is pelleted and resuspended in BM medium (1× MS salts, 1× B5 vitamins, 30 g/L sucrose, 500 mg/L MES, pH 5.8). The optical density at 600 nm is adjusted to 0.2 with BM medium and used for inoculation.

Explants are cocultivated with Agrobacterium for 20 min after which time the Agrobacterium suspension is removed. Hypocotyl explants are washed once in callus induction medium after which cocultivation proceeds for 48 h with gentle shaking on orbital shaker. After several washes in CIM, explants are transferred to selective shoot-inducing medium (500 mg/L AgNO2, 0.4 mg/L zeatin riboside, 2.0 mg/L benzylamino purine, 0.01 mg/L GA, 200 mg/L Timentin appropriate selection agent and 8 g/L agar added to basal medium) plates for regeneration at 24° C. Root formation is induced on root-inducing medium (0.5×MS salts, 0.5× B5 vitamins, 10 g/L sucrose, 500 mg/L MES, 0.1 mg/L indole-3-butyric acid, 200 mg/L Timentin, appropriate selection agent and S g/L agar, pH 5.8).

Plantlets are transferred to are removed from agar, gently washed, and transferred to potting soil in pots. Plants are grown in a humid environment for a week and then transferred to the greenhouse.

Example 5

Production of Hybrid Lines that are not Capable of Germinating

In previous experiments in Arabidopsis, lower levels of PHB were obtained when lines expressing individual PHB genes were crossed to produce a plant containing the entire PHB biosynthetic pathway (Nawrath, C., Y. Poirier, et al., 1994, Proc. Natl. Acad. Sci. USA 91, 12760-12764) than when multi-gene constructs containing the entire PHB biosynthetic pathway were constructed and transformed (Bohmert, K., I. et al., 2000, Planta 211, 841-845;U.S. Pat. No. 6,448,473). This observation led to the subsequent predominant use of multi-gene constructs for PHB production in plants. However, in some scenarios, it may be advantageous to insert a multi-gene pathway into the plant by crossing of lines containing portions of the pathway to produce hybrid plants in which the entire pathway has been reconstructed. This is especially the case when high levels of product in a seed compromises the ability of the seed to germinate or the resulting seedling to survive under normal soil growth conditions. Hybrid lines can be created by crossing a line containing one or more PHB genes with a line containing the other gene(s) needed to complete the PHB biosynthetic pathway. Use of lines that possess cytoplasmic male sterility (Esser, K. et al., 2006, Progress in Botany, Springer Berlin Heidelberg. 67, 31-52) with the appropriate maintainer and restorer lines allows these hybrid lines to be produced efficiently. Cytoplasmic male sterility systems are already available for some Brassicaceae species (Esser, K. et al., 2006, Progress in Botany, Springer Berlin Heidelberg. 67, 31-52). These Brassicaceae species can be used as gene sources to produce cytoplasmic male sterility systems for other oilseeds of interest such as Camelina. Cytoplasmic male sterility has also been reported upon expression of a β-ketothiolase from the chloroplast genome in tobacco (Ruiz, O. N. and H. Daniell, 2005, Plant Physiol. 138, 1232-1246). Male sterility has also been reported upon expression of the faoA gene encoding the α-subunit of the fatty acid β-oxidation complex from Pseudomonas putida (U.S. Pat. No. 6,586,658).

High PHB producing lines that are not capable of germination can be produced using oilseed lines that possess cytoplasmic male sterility (CMS) controlled by an extranuclear genome (i.e. mitochondria or chloroplast). The male sterile line is typically maintained by crossing with a maintainer line that is genetically identical except that it possesses normal fertile cytoplasm and is therefore male fertile. Transformation of the maintainer line with one or more genes for the PHB biosynthetic pathway and crossing this modified maintainer line with the original male sterile line will produce a male sterile line possessing a portion of the PHB biosynthetic pathway. In this example, insertion of the phaA and phaC genes into the maintainer line and crossing with the original male cytoplasmic sterile line will form a male sterile line containing the phaA and phaC genes.

Fertility can be restored to this line using a “restorer line” that carries the appropriate nuclear restorer genes. Alternatively, the restorer line can be transformed with the remaining genes required to complete the PHB biosynthetic pathway and crossed with the previously created male sterile line containing phaA and phaC to produce a hybrid line containing the entire PHB biosynthetic pathway.

Crosses can be performed in the field by planting multiple rows of the male sterile line, the line that will produce the seed, next to a few rows of the male fertile line. Harvested seed can be used for subsequent plantings or as the PHB containing seed for crushing and extraction. When expression cassettes for the PHB genes in this example are controlled by strong promoters, such as the soybean oleosin promoter, high PHB producing seeds generated in this manner will possess weak seedlings upon germination and will not be able to survive field conditions under normal growth circumstances unless treated with a material that promotes seedling strength/vigor. This adds a level of gene containment.

Cytoplasmic male sterility systems are already available for some Brassicaceae species (Esser, K., 2006, Progress in Botany, Springer Berlin Heidelberg. 67, 31-52). These Brassicaceae species can be used as gene sources to produce cytoplasmic male sterility systems for other oilseeds of interest such as Camelina. Cytoplasmic male sterility has also been reported upon expression of a β-ketothiolase from the chloroplast genome in tobacco (Ruiz, O. N. and H. Daniell, 2005, Plant Physiol. 138, 1232-1246). Overexpression of β-ketothiolase in Camelina to generate a male sterile line and subsequent crossing with a line expressing phaB and phaC could also be used for hybrid seed production.

Male sterile lines have also been produced in Brassica napus by overexpression of the faoA gene from Pseudomonas putida under the control of the phaseolin promoter sequence (U.S. Pat. No. 6,586,658).

Double haploid technology can be used to speed up the breeding process. In the double haploid technique, immature pollen grains (haploids) are exposed to treatments that result in doubling of the existing genetic material resulting in homozygous, true breeding material in a single generation.

The references, patents, and patent applications cited throughout are incorporated by reference where permissible in their entireties.

Vector: pMBXS490
(SEQ ID NO: 1)

1	GGGGATCCGT ACGTAAGTAC GTACTCAAAA TGCCAACAAA TAAAAAAAAA
51	GTTGCTTTAA TAATGCCAAA ACAAATTAAT AAAACACTTA CAACACCGGA
101	TTTTTTTTAA TTAAAATGTG CCATTTAGGA TAAATAGTTA ATATTTTTAA
151	TAATTATTTA AAAAGCCGTA TCTACTAAAA TGATTTTTAT TTGGTTGAAA
201	ATATTAATAT GTTTAAATCA ACACAATCTA TCAAAATTAA ACTAAAAAAA
251	AAATAAGTGT ACGTGGTTAA CATTAGTACA GTAATATAAG AGGAAAATGA
301	GAAATTAAGA AATTGAAAGC GAGTCTAATT TTTAAATTAT GAACCTGCAT
351	ATATAAAAGG AAAGAAAGAA TCCAGGAAGA AAAGAAATGA AACCATGCAT
401	GGTCCCCTCG TCATCACGAG TTTCTGCCAT TTGCAATAGA AACACTGAAA
451	CACCTTTCTC TTTGTCACTT AATTGAGATG CCGAAGCCAC CTCACACCAT
501	GAACTTCATG AGGTGTAGCA CCCAAGGCTT CCATAGCCAT GCATACTGAA
551	GAATGTCTCA AGCTCAGCAC CCTACTTCTG TGACGTGTCC CTCATTCACC
601	TTCCTCTCTT CCCTATAAAT AACCACGCCT CAGGTTCTCC GCTTCACAAC
651	TCAAACATTC TCTCCATTGG TCCTTAAACA CTCATCAGTC ATCACCGCGG
701	CCGCGGAATT CATGGCTTCT ATGATATCCT CTTCCGCTGT GACAACAGTC
751	AGCCGTGCCT CTAGGGGGCA ATCCGCCGCA GTGGCTCCAT TCGGCGGCCT
801	CAAATCCATG ACTGGATTCC CAGTGAAGAA GGTCAACACT GACATTACTT
851	CCATTACAAG CAATGGTGGA AGAGTAAAGT GCATGCAGGT GTGGCCTCCA
901	ATTGGAAAGA AGAAGTTTGA GACTCTTTCC TATTTGCCAC CATTGACGAG
951	AGATTCTAGA GTGACTGACG TTGTCATCGT ATCCGCCGCC CGCACCGCGG
1001	TCGGCAAGTT TGGCGGCTCG CTGGCCAAGA TCCCGGCACC GGAACTGGGT
1051	GCCGTGGTCA TCAAGGCCGC GCTGGAGCGC GCCGGCGTCA AGCCGGAGCA
1101	GGTGAGCGAA GTCATCATGG GCCAGGTGCT GACCGCCGGT TCGGGCCAGA
1151	ACCCCGCACG CCAGGCCGCG ATCAAGGCCG GCCTGCCGGC GATGGTGCCG
1201	GCCATGACCA TCAACAAGGT GTGCGGCTCG GGCCTGAAGG CCGTGATGCT
1251	GGCCGCCAAC GCGATCATGG CGGGCGACGC CGAGATCGTG GTGGCCGGCG
1301	GCCAGGAAAA CATGAGCGCC GCCCCGCACG TGCTGCCGGG CTCGCGCGAT
1351	GGTTTCCGCA TGGGCGATGC CAAGCTGGTC GACACCATGA TCGTCGACGG
1401	CCTGTGGGAC GTGTACAACC AGTACCACAT GGGCATCACC GCCGAGAACG
1451	TGGCCAAGGA ATACGGCATC ACACGCGAGG CGCAGGATGA GTTCGCCGTC
1501	GGCTCGCAGA ACAAGGCCGA AGCCGCGCAG AAGGCCGGCA AGTTTGACGA
1551	AGAGATCGTC CCGGTGCTGA TCCCGCAGCG CAAGGGCGAC CCGGTGGCCT
1601	TCAAGACCGA CGAGTTCGTG CGCCAGGGCG CCACGCTGGA CAGCATGTCC
1651	GGCCTCAAGC CCGCCTTCGA CAAGGCCGGC ACGGTGACCG CGGCCAACGC
1701	CTCGGGCCTG AACGACGGCG CCGCCGCGGT GGTGGTGATG TCGGCGGCCA
1751	AGGCCAAGGA ACTGGGCCTG ACCCCGCTGG CCACGATCAA GAGCTATGCC
1801	AACGCCGGTG TCGATCCCAA GGTGATGGGC ATGGGCCCGG TGCCGGCCTC
1851	CAAGCGCGCC CTGTCGCGCG CCGAGTGGAC CCCGCAAGAC CTGGACCTGA
1901	TGGAGATCAA CGAGGCCTTT GCCGCGCAGG CGCTGGCGGT GCACCAGCAG
1951	ATGGGCTGGG ACACCTCCAA GGTCAATGTG AACGGCGGCG CCATCGCCAT
2001	CGGCCACCCG ATCGGCGCGT CGGGCTGCCG TATCCTGGTG ACGCTGCTGC
2051	ACGAGATGAA GCGCCGTGAC GCGAAGAAGG GCCTGGCCTC GCTGTGCATC
2101	GGCGGCGGCA TGGGCGTGGC GCTGGCAGTC GAGCGCAAAT AACTCGAGGC
2151	GGCCGCAGCC CTTTTTGTAT GTGCTACCCC ACTTTTGTCT TTTTGGCAAT
2201	AGTGCTAGCA ACCAATAAAT AATAATAATA ATAATGAATA AGAAAACAAA
2251	GGCTTTAGCT TGCCTTTTGT TCACTGTAAA ATAATAATGT AAGTACTCTC
2301	TATAATGAGT CACGAAACTT TTGCGGGAAT AAAAGGAGAA ATTCCAATGA
2351	GTTTTCTGTC AAATCTTCTT TTGTCTCTCT CTCTCTCTCT TTTTTTTTTT
2401	TCTTTCTTCT GAGCTTCTTG CAAAACAAAA GGCAAACAAT AACGATTGGT
2451	CCAATGATAG TTAGCTTGAT CGATGATATC TTTAGGAAGT GTTGGCAGGA
2501	CAGGACATGA TGTAGAAGAC TAAAATTGAA AGTATTGCAG ACCCAATAGT
2551	TGAAGATTAA CTTTAAGAAT GAAGACGTCT TATCAGGTTC TTCATGACTT
2601	AAGCTTTAAG AGGAGTCCAC CATGGTAGAT CTGACTAGTA GAAGGTAATT
2651	ATCCAAGATG TAGCATCAAG AATCCAATGT TTACGGGAAA AACTATGGAA
2701	GTATTATGTG AGCTCAGCAA GAAGCAGATC AATATGCGGC ACATATGCAA
2751	CCTATGTTCA AAAATGAAGA ATGTACAGAT ACAAGATCCT ATACTGCCAG
2801	AATACGAAGA AGAATACGTA GAAATTAAGA AAGAAGAACC AGGCGAAGAA
2851	AAGAATCTTG AAGACGTAAG CACTGACGAC AACACTGAAA AGAAGAAGAT
2901	AAGGTCGGTG ATTGTGAAAG AGACATAGAG GACACATGTA AGGTGGAAAA
2951	TGTAAGGGCG GAAAGTAACC TTATCACAAA GGAATCTTAT CCCCCACTAC
3001	TTATCCTTTT ATATTTTTCC GTGTCATTTT TGCCCTTGAG TTTTCCTATA
3051	TAAGGAACCA AGTTCGGCAT TTGTGAAAAC AAGAAAAAAT TGGTGTAAGC
3101	TATTTTCTTT GAAGTACTGA GGATACAACT TCAGAGAAAT TTGTAAGAAA
3151	GTGGATCGAA ACCATGGCCT CCTCCGAGAA CGTCATCACC GAGTTCATGC
3201	GCTTCAAGGT GCGCATGGAG GGCACCGTGA ACGGCCACGA GTTCGAGATC
3251	GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCCACAACA CCGTGAAGCT
3301	GAAGGTGACC AAGGGCGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC
3351	CCCAGTTCCA GTACGGCTCC AAGGTGTACG TGAAGCACCC CGCCGACATC
3401	CCCGACTACA AGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
3451	GATGAACTTC GAGGACGGCG GCGTGGCGAC CGTGACCCAG GACTCCTCCC
3501	TGCAGGACGG CTGCTTCATC TACAAGGTGA AGTTCATCGG CGTGAACTTC
3551	CCCTCCGACG GCCCCGTGAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC
3601	CACCGAGCGC CTGTACCCCC GCGACGGCGT GCTGAAGGGC GAGACCCACA
3651	AGGCCCTGAA GCTGAAGGAC GGCGGCCACT ACCTGGTGGA GTTCAAGTCC
3701	ATCTACATGG CCAAGAAGCC CGTGCAGCTG CCCGGCTACT ACTACGTGGA
3751	CGCCAAGCTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAGC
3801	AGTACGAGCG CACCGAGGGC CGCCACCACC TGTTCCTGGT ACCAATGAGC
3851	TCTGTCCAAC AGTCTCAGGG TTAATGTCTA TGTATCTTAA ATAATGTTGT
3901	CGGCGATCGT TCAAACATTT GGCAATAAAG TTTCTTAAGA TTGAATCCTG
3951	TTGCCGGTCT TGCGATGATT ATCATATAAT TTCTGTTGAA TTACGTTAAG
4001	CATGTAATAA TTAACATGTA ATGCATGACG TTATTTATGA GATGGGTTTT
4051	TATGATTAGA GTCCCGCAAT TATACATTTA ATACGCGATA GAAAACAAAA
4101	TATAGCGCGC AAACTAGGAT AAATTATCGC GCGCGGTGTC ATCTATGTTA
4151	CTAGATCGGG AATTAAACTA TCAGTGTTTG ACAGGATATA TTGGCGGGTA
4201	AACCTAAGAG AAAAGAGCGT TTATTAGAAT AACGGATATT TAAAAGGGCG
4251	TGAAAAGGTT TATCCGTTCG TCCATTTGTA TGTGCATGCC AACCACAGGG
4301	TTCCCCTCGG GATCAAAGTA CTTTGATCCA ACCCCTCCGC TGCTATAGTG
4351	CAGTCGGCTT CTGACGTTCA GTGCAGCCGT CTTCTGAAAA CGACATGTCG
4401	CACAAGTCCT AAGTTACGCG ACAGGCTGCC GCCCTGCCCT TTTCCTGGCG
4451	TTTTCTTGTC GCGTGTTTTA GTCGCATAAA GTAGAATACT TGCGACTAGA
4501	ACCGGAGACA TTACGCCATG AACAAGAGCG CCGCCGCTGG CCTGCTGGGC
4551	TATGCCCGCG TCAGCACCGA CGACCAGGAC TTGACCAACC AACGGGCCGA
4601	ACTGCACGCG GCCGGCTGCA CCAAGCTGTT TTCCGAGAAG ATCACCGGCA
4651	CCAGGCGCGA CCGCCCGGAG CTGGCCAGGA TGCTTGACCA CCTACGCCCT
4701	GGCGACGTTG TGACAGTGAC CAGGCTAGAC CGCCTGGCCC GCAGCACCCG
4751	CGACCTACTG GACATTGCCG AGCGCATCCA GGAGGCCGGC GCGGGCCTGC
4801	GTAGCCTGGC AGAGCCGTGG GCCGACACCA CCACGCCGGC CGGCCGCATG
4851	GTGTTGACCG TGTTCGCCGG CATTGCCGAG TTCGAGCGTT CCCTAATCAT
4901	CGACCGCACC CGGAGCGGGC GCGAGGCCGC CAAGGCCCGA GGCGTGAAGT
4951	TTGGCCCCCG CCCTACCCTC ACCCCGGCAC AGATCGCGCA CGCCCGCGAG
5001	CTGATCGACC AGGAAGGCCG CACCGTGAAA GAGGCGGCTG CACTGCTTGG
5051	CGTGCATCGC TCGACCCTGT ACCGCGCACT TGAGCGCAGC GAGGAAGTGA
5101	CGCCCACCGA GGCCAGGCGG CGCGGTGCCT TCCGTGAGGA CGCATTGACC
5151	GAGGCCGACG CCCTGGCGGC CGCCGAGAAT GAACGCCAAG AGGAACAAGC
5201	ATGAAACCGC ACCAGGACGG CCAGGACGAA CCGTTTTTCA TTACCGAAGA
5251	GATCGAGGCG GAGATGATCG CGGCCGGGTA CGTGTTCGAG CCGCCCGCGC
5301	ACGTCTCAAC CGTGCGGCTG CATGAAATCC TGGCCGGTTT GTCTGATGCC
5351	AAGCTGGCGG CCTGGCCGGC CAGCTTGGCC GCTGAAGAAA CCGAGCGCCG
5401	CCGTCTAAAA AGGTGATGTG TATTTGAGTA AAACAGCTTG CGTCATGCGG
5451	TCGCTGCGTA TATGATGCGA TGAGTAAATA AACAAATACG CAAGGGGAAC
5501	GCATGAAGGT TATCGCTGTA CTTAACCAGA AAGGCGGGTC AGGCAAGACG
5551	ACCATCGCAA CCCATCTAGC CCGCGCCCTG CAACTCGCCG GGGCCGATGT
5601	TCTGTTAGTC GATTCCGATC CCCAGGGCAG TGCCCGCGAT TGGGCGGCCG
5651	TGCGGGAAGA TCAACCGCTA ACCGTTGTCG GCATCGACCG CCCGACGATT
5701	GACCGCGACG TGAAGGCCAT CGGCCGGCGC GACTTCGTAG TGATCGACGG
5751	AGCGCCCCAG GCGGCGGACT TGGCTGTGTC CGCGATCAAG GCAGCCGACT
5801	TCGTGCTGAT TCCGGTGCAG CCAAGCCCTT ACGACATATG GGCCACCGCC
5851	GACCTGGTGG AGCTGGTTAA GCAGCGCATT GAGGTCACGG ATGGAAGGCT
5901	ACAAGCGGCC TTTGTCGTGT CGCGGGCGAT CAAAGGCACG CGCATCGGCG
5951	GTGAGGTTGC CGAGGCGCTG GCCGGGTACG AGCTGCCCAT TCTTGAGTCC
6001	CGTATCACGC AGCGCGTGAG CTACCCAGGC ACTGCCGCCG CCGGCACAAC
6051	CGTTCTTGAA TCAGAACCCG AGGGCGACGC TGCCCGCGAG GTCCAGGCGC
6101	TGGCCGCTGA AATTAAATCA AAACTCATTT GAGTTAATGA GGTAAAGAGA
6151	AAATGAGCAA AAGCACAAAC ACGCTAAGTG CCGGCCGTCC GAGCGCACGC
6201	AGCAGCAAGG CTGCAACGTT GGCCAGCCTG GCAGACACGC CAGCCATGAA
6251	GCGGGTCAAC TTTCAGTTGC CGGCGGAGGA TCACACCAAG CTGAAGATGT
6301	ACGCGGTACG CCAAGGCAAG ACCATTACCG AGCTGCTATC TGAATACATC
6351	GCGCAGCTAC CAGAGTAAAT GAGCAAATGA ATAAATGAGT AGATGAATTT
6401	TAGCGGCTAA AGGAGGCGGC ATGGAAAATC AAGAACAACC AGGCACCGAC
6451	GCCGTGGAAT GCCCCATGTG TGGAGGAACG GGCGGTTGGC CAGGCGTAAG
6501	CGGCTGGGTT GTCTGCCGGC CCTGCAATGG CACTGGAACC CCCAAGCCCG
6551	AGGAATCGGC GTGACGGTCG CAAACCATCC GGCCCGGTAC AAATCGGCGC
6601	GGCGCTGGGT GATGACCTGG TGGAGAAGTT GAAGGCCGCG CAGGCCGCCC
6651	AGCGGCAACG CATCGAGGCA GAAGCACGCC CCGGTGAATC GTGGCAAGCG
6701	GCCGCTGATC GAATCCGCAA AGAATCCCGG CAACCGCCGG CAGCCGGTGC
6751	GCCGTCGATT AGGAAGCCGC CCAAGGGCGA CGAGCAACCA GATTTTTTCG
6801	TTCCGATGCT CTATGACGTG GGCACCCGCG ATAGTCGCAG CATCATGGAC
6851	GTGGCCGTTT TCCGTCTGTC GAAGCGTGAC CGACGAGCTG GCGAGGTGAT
6901	CCGCTACGAG CTTCCAGACG GGCACGTAGA GGTTTCCGCA GGGCCGGCCG
6951	GCATGGCCAG TGTGTGGGAT TACGACCTGG TACTGATGGC GGTTTCCCAT
7001	CTAACCGAAT CCATGAACCG ATACCGGGAA GGGAAGGGAG ACAAGCCCGG
7051	CCGCGTGTTC CGTCCACACG TTGCGGACGT ACTCAAGTTC TGCCGGCGAG
7101	CCGATGGCGG AAAGCAGAAA GACGACCTGG TAGAAACCTG CATTCGGTTA
7151	AACACCACGC ACGTTGCCAT GCAGCGTACG AAGAAGGCCA AGAACGGCCG
7201	CCTGGTGACG GTATCCGAGG GTGAAGCCTT GATTAGCCGC TACAAGATCG
7251	TAAAGAGCGA AACCGGGCGG CCGGAGTACA TCGAGATCGA GCTAGCTGAT
7301	TGGATGTACC GCGAGATCAC AGAAGGCAAG AACCCGGACG TGCTGACGGT
7351	TCACCCCGAT TACTTTTTGA TCGATCCCGG CATCGGCCGT TTTCTCTACC
7401	GCCTGGCACG CCGCGCCGCA GGCAAGGCAG AAGCCAGATG GTTGTTCAAG
7451	ACGATCTACG AACGCAGTGG CAGCGCCGGA GAGTTCAAGA AGTTCTGTTT
7501	CACCGTGCGC AAGCTGATCG GGTCAAATGA CCTGCCGGAG TACGATTTGA
7551	AGGAGGAGGC GGGGCAGGCT GGCCCGATCC TAGTCATGCG CTACCGCAAC
7601	CTGATCGAGG GCGAAGCATC CGCCGGTTCC TAATGTACGG AGCAGATGCT
7651	AGGGCAAATT GCCCTAGCAG GGGAAAAAGG TCGAAAAGGT CTCTTTCCTG
7701	TGGATGTACC GTACATTGGG AACCCAAAGC CGTACATTGG GAACCGGAAC
7751	CCGTACATTG GGAACCCAAA GCCGTACATT GGGAACCGGT CACACATGTA
7801	AGTGACTGAT ATAAAAGAGA AAGAAGGCCA TTTTTCCGCC TAAAACTCTT
7851	TAAAACTTAT TAAAACTCTT AAAACCCGCC TGGCCTGTGC ATAACTGTCT
7901	GGCCAGCGCA CAGCCGAAGA GCTGCAAAAA GCGCCTACCC TTCGGTCGCT
7951	GCGCTCCCTA CGCCCCGCCG CTTCGCGTCG GCCTATCGCG GCCGCTGGCC
8001	GCTCAAAAAT GGCTGGCCTA CGGCCAGGCA ATCTACCAGG GCGCGGACAA
8051	GCCGCGCCGT CGCCACTCGA CCGCCGGCGC CCACATCAAG GCACCCTGCC
8101	TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG
8151	GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG
8201	TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC
8251	AGTCACGTAG CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG
8301	AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA
8351	TGCGTAAGGA GAAAATACCG CATCAGGCGC TCTTCCGCTT CCTCGCTCAC
8401	TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
8451	CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
8501	AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
8551	GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
8601	ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
8651	CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
8701	GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
8751	TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
8801	TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
8851	CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
8901	CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
8951	GGCTGGCCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
9001	AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
9051	AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
9101	GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
9151	AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
9201	ACTCACGTTA AGGGATTTTG GTCATGCATT CTAGGTACTA AAACAATTCA
9251	TCCAGTAAAA TATAATATTT TATTTTCTCC CAATCAGGCT TGATCCCCAG
9301	TAAGTCAAAA AATAGCTCGA CATACTGTTC TTCCCCGATA TCCTCCCTGA
9351	TCGACCGGAC GCAGAAGGCA ATGTCATACC ACTTGTCCGC CCTGCCGCTT
9401	CTCCCAAGAT CAATAAAGCC ACTTACTTTG CCATCTTTCA CAAAGATGTT
9451	GCTGTCTCCC AGGTCGCCGT GGGAAAAGAC AAGTTCCTCT TCGGGCTTTT
9501	CCGTCTTTAA AAAATCATAC AGCTCGCGCG GATCTTTAAA TGGAGTGTCT
9551	TCTTCCCAGT TTTCGCAATC CACATCGGCC AGATCGTTAT TCAGTAAGTA
9601	ATCCAATTCG GCTAAGCGGC TGTCTAAGCT ATTCGTATAG GGACAATCCG
9651	ATATGTCGAT GGAGTGAAAG AGCCTGATGC ACTCCGCATA CAGCTCGATA
9701	ATCTTTTCAG GGCTTTGTTC ATCTTCATAC TCTTCCGAGC AAAGGACGCC
9751	ATCGGCCTCA CTCATGAGCA GATTGCTCCA GCCATCATGC CGTTCAAAGT
9801	GCAGGACCTT TGGAACAGGC AGCTTTCCTT CCAGCCATAG CATCATGTCC
9851	TTTTCCCGTT CCACATCATA GGTGGTCCCT TTATACCGGC TGTCCGTCAT
9901	TTTTAAATAT AGGTTTTCAT TTTCTCCCAC CAGCTTATAT ACCTTAGCAG
9951	GAGACATTCC TTCCGTATCT TTTACGCAGC GGTATTTTTC GATCAGTTTT
10001	TTCAATTCCG GTGATATTCT CATTTTAGCC ATTTATTATT TCCTTCCTCT
10051	TTTCTACAGT ATTTAAAGAT ACCCCAAGAA GCTAATTATA ACAAGACGAA
10101	CTCCAATTCA CTGTTCCTTG CATTCTAAAA CCTTAAATAC CAGAAAACAG
10151	CTTTTTCAAA GTTGTTTTCA AAGTTGGCGT ATAACATAGT ATCGACGGAG
10201	CCGATTTTGA AACCGCGGTG ATCACAGGCA GCAACGCTCT GTCATCGTTA
10251	CAATCAACAT GCTACCCTCC GCGAGATCAT CCGTGTTTCA AACCCGGCAG
10301	CTTAGTTGCC GTTCTTCCGA ATAGCATCGG TAACATGAGC AAAGTCTGCC
10351	GCCTTACAAC GGCTCTCCCG CTGACGCCGT CCCGGACTGA TGGGCTGCCT
10401	GTATCGAGTG GTGATTTTGT GCCGAGCTGC CGGTCGGGGA GCTGTTGGCT
10451	GGCTGGTGGC AGGATATATT GTGGTGTAAA CAAATTGACG CTTAGACAAC
10501	TTAATAACAC ATTGCGGACG TTTTTAATGT ACTGAATTAA CGCCGAATTA
10551	ATTCCTAGGC CACCATGTTG GGCCCGGGGC GCGCCGTACG TAGTGTTTAT
10601	CTTTGTTGCT TTTCTGAACA ATTTATTTAC TATGTAAATA TATTATCAAT
10651	GTTTAATCTA TTTTAATTTG CACATGAATT TTCATTTTAT TTTTACTTTA
10701	CAAAACAAAT AAATATATAT GCAAAAAAAT TTACAAACGA TGCACGGGTT
10751	ACAAACTAAT TTCATTAAAT GCTAATGCAG ATTTTGTGAA GTAAAACTCC
10801	AATTATGATG AAAAATACCA CCAACACCAC CTGCGAAACT GTATCCCAAC
10851	TGTCCTTAAT AAAAATGTTA AAAAGTATAT TATTCTCATT TGTCTGTCAT
10901	AATTTATGTA CCCCACTTTA ATTTTTCTGA TGTACTAAAC CGAGGGCAAA
10951	CTGAAACCTG TTCCTCATGC AAAGCCCCTA CTCACCATGT ATCATGTACG
11001	TGTCATCACC CAACAACTCC ACTTTTGCTA TATAACAACA CCCCCGTCAC
11051	ACTCTCCCTC TCTAACACAC ACCCCACTAA CAATTCCTTC ACTTGCAGCA
11101	CTGTTGCATC ATCATCTTCA TTGCAAAACC CTAAACTTCA CCTTCAACCG
11151	CGGCCGCATG GCTTCTATGA TATCCTCTTC CGCTGTGACA ACAGTCAGCC
11201	GTGCCTCTAG GGGGCAATCC GCCGCAGTGG CTCCATTCGG CGGCCTCAAA
11251	TCCATGACTG GATTCCCAGT GAAGAAGGTC AACACTGACA TTACTTCCAT
11301	TACAAGCAAT GGTGGAAGAG TAAAGTGCAT GCAGGTGTGG CCTCCAATTG
11351	GAAAGAAGAA GTTTGAGACT CTTTCCTATT TGCCACCATT GACGAGAGAT
11401	TCTAGAGTGA GTAACAAGAA CAACGATGAG CTGCAGTGGC AATCCTGGTT
11451	CAGCAAGGCG CCCACCACCG AGGCGAACCC GATGGCCACC ATGTTGCAGG
11501	ATATCGGCGT TGCGCTCAAA CCGGAAGCGA TGGAGCAGCT GAAAAACGAT
11551	TATCTGCGTG ACTTCACCGC GTTGTGGCAG GATTTTTTGG CTGGCAAGGC
11601	GCCAGCCGTC AGCGACCGCC GCTTCAGCTC GGCAGCCTGG CAGGGCAATC
11651	CGATGTCGGC CATCATGTCC GCATCTTACC TGCTCAACGC CAAATTCCTC
11701	AGTGCCATGG TGGAGGCGGT GGACACCGCA CCCCAGCAAA AGCAGAAAAT
11751	ACGCTTTGCC GTGCAGCAGG TGATTGATGC CATGTCGCCC GCGAACTTCC
11801	TCGCCACCAA CCCGGAAGCG CAGCAAAAAC TGATTGAAAC CAAGGGCGAG
11851	AGCCTGACGC GTGGCCTGGT CAATATGCTG GGCGATATCA ACAAGGGCCA
11901	TATCTCGCTG TCGGACGAAT CGGCCTTTGA AGTGGGCCGC AACCTGGCCA
11951	TTACCCCGGG CACCGTGATT TACGAAAATC CGCTGTTCCA GCTGATCCAG
12001	TACACGCCGA CCACGCCGAC GGTCAGCCAG CGCCCGCTGT TGATGGTGCC
12051	GCCGTGCATC AACAAGTTCT ACATCCTCGA CCTTCAACCG GAAAATTCGC
12101	TGGTGCGCTA CGCGGTGGAG CAGGGCAACA CCGTGTTCCT GATCTCGTGG
12151	AGCAATCCGG ACAAGTCGCT GGCCGGCACC ACCTGGGACG ACTACGTGGA
12201	GCAGGGCGTG ATCGAAGCGA TCCGCATCGT CCAGGACGTC AGCGGCCAGG
12251	ACAAGCTGAA CATGTTCGGC TTCTGCGTGG GCGGCACCAT CGTTGCCACC
12301	GCACTGGCGG TACTGGCGGC GCGTGGCCAG CACCCGGCGG CCAGCCTGAC
12351	CCTGCTGACC ACCTTCCTCG ACTTCAGCGA CACCGGCGTG CTCGACGTCT
12401	TCGTCGATGA AACCCAGGTC GCGCTGCGTG AACAGCAATT GCGCGATGGC
12451	GGCCTGATGC CGGGCCGTGA CCTGGCCTCG ACCTTCTCGA GCCTGCGTCC
12501	GAACGACCTG GTATGGAACT ATGTGCAGTC GAACTACCTC AAAGGCAATG
12551	AGCCGGCGGC GTTTGACCTG CTGTTCTGGA ATTCGGACAG CACCAATTTG
12601	CCGGGCCCGA TGTTCTGCTG GTACCTGCGC AACACCTACC TGGAAAACAG
12651	CCTGAAAGTG CCGGGCAAGC TGACGGTGGC CGGCGAAAAG ATCGACCTCG
12701	GCCTGATCGA CGCCCCGGCC TTCATCTACG GTTCGCGCGA AGACCACATC
12751	GTGCCGTGGA TGTCGGCGTA CGGTTCGCTC GACATCCTCA ACCAGGGCAA
12801	GCCGGGCGCC AACCGCTTCG TGCTGGGCGC GTCCGGCCAT ATCGCCGGCG
12851	TGATCAACTC GGTGGCCAAG AACAAGCGCA GCTACTGGAT CAACGACGGT
12901	GGCGCCGCCG ATGCCCAGGC CTGGTTCGAT GGCGCGCAGG AAGTGCCGGG
12951	CAGCTGGTGG CCGCAATGGG CCGGGTTCCT GACCCAGCAT GGCGGCAAGA
13001	AGGTCAAGCC CAAGGCCAAG CCCGGCAACG CCCGCTACAC CGCGATCGAG
13051	GCGGCGCCCG GCCGTTACGT CAAAGCCAAG GGCTGAGCGG CCGCTGAGTA
13101	ATTCTGATAT TAGAGGGAGC ATTAATGTGT TGTTGTGATG TGGTTTATAT
13151	GGGGAAATTA AATAAATGAT GTATGTACCT CTTGCCTATG TAGGTTTGTG
13201	TGTTTTGTTT TGTTGTCTAG CTTTGGTTAT TAAGTAGTAG GGACGTTCGT
13251	TCGTGTCTCA AAAAAAGGGG TACTACCACT CTGTAGTGTA TATGGATGCT
13301	GGAAATCAAT GTGTTTTGTA TTTGTTCACC TCCATTGTTG AATTCAATGT
13351	CAAATGTGTT TTGCGTTGGT TATGTGTAAA ATTACTATCT TTCTCGTCCG
13401	ATGATCAAAG TTTTAAGCAA CAAAACCAAG GGTGAAATTT AAACTGTGCT
13451	TTGTTGAAGA TTCTTTTATC ATATTGAAAA TCAAATTACT AGCAGCAGAT
13501	TTTACCTAGC ATGAAATTTT ATCAACAGTA CAGCACTCAC TAACCAAGTT
13551	CCAAACTAAG ATGCGCCATT AACATCAGCC AATAGGCATT TTCAGCAAGG
13601	CGCGCCCGCG CCGATGTATG TGACAACCCT CGGGATTGTT GATTTATTTC
13651	AAAACTAAGA GTTTTTGTCT TATTGTTCTC GTCTATTTTG GATATCAATC
13701	TTAGTTTTAT ATCTTTTCTA GTTCTCTACG TGTTAAATGT TCAACACACT
13751	AGCAATTTGG CCTGCCAGCG TATGGATTAT GGAACTATCA AGTCTGTGAC
13801	GCGCCGTACG TAGTGTTTAT CTTTGTTGCT TTTCTGAACA ATTTATTTAC
13851	TATGTAAATA TATTATCAAT GTTTAATCTA TTTTAATTTG CACATGAATT
13901	TTCATTTTAT TTTTACTTTA CAAAACAAAT AAATATATAT GCAAAAAAAT
13951	TTACAAACGA TGCACGGGTT ACAAACTAAT TTCATTAAAT GCTAATGCAG
14001	ATTTTGTGAA GTAAAACTCC AATTATGATG AAAAATACCA CCAACACCAC
14051	CTGCGAAACT GTATCCCAAC TGTCCTTAAT AAAAATGTTA AAAAGTATAT
14101	TATTCTCATT TGTCTGTCAT AATTTATGTA CCCCACTTTA ATTTTTCTGA
14151	TGTACTAAAC CGAGGGCAAA CTGAAACCTG TTCCTCATGC AAAGCCCCTA
14201	CTCACCATGT ATCATGTACG TGTCATCACC CAACAACTCC ACTTTTGCTA
14251	TATAACAACA CCCCCGTCAC ACTCTCCCTC TCTAACACAC ACCCCACTAA
14301	CAATTCCTTC ACTTGCAGCA CTGTTGCATC ATCATCTTCA TTGCAAAACC
14351	CTAAACTTCA CCTTCAACCG CGGCCGCATG GCTTCTATGA TATCCTCTTC
14401	CGCTGTGACA ACAGTCAGCC GTGCCTCTAG GGGGCAATCC GCCGCAGTGG
14451	CTCCATTCGG CGGCCTCAAA TCCATGACTG GATTCCCAGT GAAGAAGGTC
14501	AACACTGACA TTACTTCCAT TACAAGCAAT GGTGGAAGAG TAAAGTGCAT
14551	GCAGGTGTGG CCTCCAATTG GAAAGAAGAA GTTTGAGACT CTTTCCTATT
14601	TGCCACCATT GACGAGAGAT TCTAGAGTGA CTCAGCGCAT TGCGTATGTG
14651	ACCGGCGGCA TGGGTGGTAT CGGAACCGCC ATTTGCCAGC GGCTGGCCAA
14701	GGATGGCTTT CGTGTGGTGG CCGGTTGCGG CCCCAACTCG CCGCGCCGCG
14751	AAAAGTGGCT GGAGCAGCAG AAGGCCCTGG GCTTCGATTT CATTGCCTCG
14801	GAAGGCAATG TGGCTGACTG GGACTCGACC AAGACCGCAT TCGACAAGGT
14851	CAAGTCCGAG GTCGGCGAGG TTGATGTGCT GATCAACAAC GCCGGTATCA
14901	CCCGCGACGT GGTGTTCCGC AAGATGACCC GCGCCGACTG GGATGCGGTG
14951	ATCGACACCA ACCTGACCTC GCTGTTCAAC GTCACCAAGC AGGTGATCGA
15001	CGGCATGGCC GACCGTGGCT GGGGCCGCAT CGTCAACATC TCGTCGGTGA
15051	ACGGGCAGAA GGGCCAGTTC GGCCAGACCA ACTACTCCAC CGCCAAGGCC
15101	GGCCTGCATG GCTTCACCAT GGCACTGGCG CAGGAAGTGG CGACCAAGGG
15151	CGTGACCGTC AACACGGTCT CTCCGGGCTA TATCGCCACC GACATGGTCA
15201	AGGCGATCCG CCAGGACGTG CTCGACAAGA TCGTCGCGAC GATCCCGGTC
15251	AAGCGCCTGG GCCTGCCGGA AGAGATCGCC TCGATCTGCG CCTGGTTGTC
15301	GTCGGAGGAG TCCGGTTTCT CGACCGGCGC CGACTTCTCG CTCAACGGCG
15351	GCCTGCATAT GGGCTGAGCG GCCGCTGAGT AATTCTGATA TTAGAGGGAG
15401	CATTAATGTG TTGTTGTGAT GTGGTTTATA TGGGGAAATT AAATAAATGA
15451	TGTATGTACC TCTTGCCTAT GTAGGTTTGT GTGTTTTGTT TTGTTGTCTA
15501	GCTTTGGTTA TTAAGTAGTA GGGACGTTCG TTCGTGTCTC AAAAAAAGGG
15551	GTACTACCAC TCTGTAGTGT ATATGGATGC TGGAAATCAA TGTGTTTTGT
15601	ATTTGTTCAC CTCCATTGTT GAATTCAATG TCAAATGTGT TTTGCGTTGG
15651	TTATGTGTAA AATTACTATC TTTCTCGTCC GATGATCAAA GTTTTAAGCA
15701	ACAAAACCAA GGGTGAAATT TAAACTGTGC TTTGTTGAAG ATTCTTTTAT
15751	CATATTGAAA ATCAAATTAC TAGCAGCAGA TTTTACCTAG CATGAAATTT
15801	TATCAACAGT ACAGCACTCA CTAACCAAGT TCCAAACTAA GATGCGCCAT
15851	TAACATCAGC CAATAGGCAT TTTCAGCAAG GCGCGTAA

pMBXS364
(SEQ ID NO: 2)

1	CATGCCAACC ACAGGGTTCC CCTCGGGATC AAAGTACTTT GATCCAACCC
51	CTCCGCTGCT ATAGTGCAGT CGGCTTCTGA CGTTCAGTGC AGCCGTCTTC
101	TGAAAACGAC ATGTCGCACA AGTCCTAAGT TACGCGACAG GCTGCCGCCC
151	TGCCCTTTTC CTGGCGTTTT CTTGTCGCGT GTTTTAGTCG CATAAAGTAG
201	AATACTTGCG ACTAGAACCG GAGACATTAC GCCATGAACA AGAGCGCCGC
251	CGCTGGCCTG CTGGGCTATG CCCGCGTCAG CACCGACGAC CAGGACTTGA
301	CCAACCAACG GGCCGAACTG CACGCGGCCG GCTGCACCAA GCTGTTTTCC
351	GAGAAGATCA CCGGCACCAG GCGCGACCGC CCGGAGCTGG CCAGGATGCT
401	TGACCACCTA CGCCCTGGCG ACGTTGTGAC AGTGACCAGG CTAGACCGCC
451	TGGCCCGCAG CACCCGCGAC CTACTGGACA TTGCCGAGCG CATCCAGGAG
501	GCCGGCGCGG GCCTGCGTAG CCTGGCAGAG CCGTGGGCCG ACACCACCAC
551	GCCGGCCGGC CGCATGGTGT TGACCGTGTT CGCCGGCATT GCCGAGTTCG
601	AGCGTTCCCT AATCATCGAC CGCACCCGGA GCGGGCGCGA GGCCGCCAAG
651	GCCCGAGGCG TGAAGTTTGG CCCCCGCCCT ACCCTCACCC CGGCACAGAT
701	CGCGCACGCC CGCGAGCTGA TCGACCAGGA AGGCCGCACC GTGAAAGAGG
751	CGGCTGCACT GCTTGGCGTG CATCGCTCGA CCCTGTACCG CGCACTTGAG
801	CGCAGCGAGG AAGTGACGCC CACCGAGGCC AGGCGGCGCG GTGCCTTCCG
851	TGAGGACGCA TTGACCGAGG CCGACGCCCT GGCGGCCGCC GAGAATGAAC
901	GCCAAGAGGA ACAAGCATGA AACCGCACCA GGACGGCCAG GACGAACCGT
951	TTTTCATTAC CGAAGAGATC GAGGCGGAGA TGATCGCGGC CGGGTACGTG
1001	TTCGAGCCGC CCGCGCACGT CTCAACCGTG CGGCTGCATG AAATCCTGGC
1051	CGGTTTGTCT GATGCCAAGC TGGCGGCCTG GCCGGCCAGC TTGGCCGCTG
1101	AAGAAACCGA GCGCCGCCGT CTAAAAAGGT GATGTGTATT TGAGTAAAAC
1151	AGCTTGCGTC ATGCGGTCGC TGCGTATATG ATGCGATGAG TAAATAAACA
1201	AATACGCAAG GGGAACGCAT GAAGGTTATC GCTGTACTTA ACCAGAAAGG
1251	CGGGTCAGGC AAGACGACCA TCGCAACCCA TCTAGCCCGC GCCCTGCAAC
1301	TCGCCGGGGC CGATGTTCTG TTAGTCGATT CCGATCCCCA GGGCAGTGCC
1351	CGCGATTGGG CGGCCGTGCG GGAAGATCAA CCGCTAACCG TTGTCGGCAT
1401	CGACCGCCCG ACGATTGACC GCGACGTGAA GGCCATCGGC CGGCGCGACT
1451	TCGTAGTGAT CGACGGAGCG CCCCAGGCGG CGGACTTGGC TGTGTCCGCG
1501	ATCAAGGCAG CCGACTTCGT GCTGATTCCG GTGCAGCCAA GCCCTTACGA
1551	CATATGGGCC ACCGCCGACC TGGTGGAGCT GGTTAAGCAG CGCATTGAGG
1601	TCACGGATGG AAGGCTACAA GCGGCCTTTG TCGTGTCGCG GGCGATCAAA
1651	GGCACGCGCA TCGGCGGTGA GGTTGCCGAG GCGCTGGCCG GGTACGAGCT
1701	GCCCATTCTT GAGTCCCGTA TCACGCAGCG CGTGAGCTAC CCAGGCACTG
1751	CCGCCGCCGG CACAACCGTT CTTGAATCAG AACCCGAGGG CGACGCTGCC
1801	CGCGAGGTCC AGGCGCTGGC CGCTGAAATT AAATCAAAAC TCATTTGAGT
1851	TAATGAGGTA AAGAGAAAAT GAGCAAAAGC ACAAACACGC TAAGTGCCGG
1901	CCGTCCGAGC GCACGCAGCA GCAAGGCTGC AACGTTGGCC AGCCTGGCAG
1951	ACACGCCAGC CATGAAGCGG GTCAACTTTC AGTTGCCGGC GGAGGATCAC
2001	ACCAAGCTGA AGATGTACGC GGTACGCCAA GGCAAGACCA TTACCGAGCT
2051	GCTATCTGAA TACATCGCGC AGCTACCAGA GTAAATGAGC AAATGAATAA
2101	ATGAGTAGAT GAATTTTAGC GGCTAAAGGA GGCGGCATGG AAAATCAAGA
2151	ACAACCAGGC ACCGACGCCG TGGAATGCCC CATGTGTGGA GGAACGGGCG
2201	GTTGGCCAGG CGTAAGCGGC TGGGTTGTCT GCCGGCCCTG CAATGGCACT
2251	GGAACCCCCA AGCCCGAGGA ATCGGCGTGA CGGTCGCAAA CCATCCGGCC
2301	CGGTACAAAT CGGCGCGGCG CTGGGTGATG ACCTGGTGGA GAAGTTGAAG
2351	GCCGCGCAGG CCGCCCAGCG GCAACGCATC GAGGCAGAAG CACGCCCCGG
2401	TGAATCGTGG CACGCGGCCG CTGATCGAAT CCGCAAAGAA TCCCGGCAAC
2451	CGCCGGCAGC CGGTGCGCCG TCGATTAGGA AGCCGCCCAA GGGCGACGAG
2501	CAACCAGATT TTTTCGTTCC GATGCTCTAT GACGTGGGCA CCCGCGTCAG
2551	TCGCAGCATC ATGGACGTGG CCGTTTTCCG TCTGTCGAAG CGTGACCGAC
2601	GAGCTGGCGA GGTGATCCGC TACGAGCTTC CAGACGGGCA CGTAGAGGTT
2651	TCCGCAGGGC CGGCCGGCAT GGCCAGTGTG TGGGATTACG ACCTGGTACT
2701	GATGGCGGTT TCCCATCTAA CCGAATCCAT GAACCGATAC CGGGAAGGGA
2751	AGGGAGACAA GCCCGGCCGC GTGTTCCGTC CACACGTTGC GGACGTACTC
2801	AAGTTCTGCC GGCGAGCCGA TGGCGGAAAG CAGAAAGACG ACCTGGTAGA
2851	AACCTGCATT CGGTTAAACA CCACGCACGT TGCCATGCAG CGTACGAAGA
2901	AGGCCAAGAA CGGCCGCCTG GTGACGGTAT CCGAGGGTGA AGCCTTGATT
2951	AGCCGCTACA AGATCGTAAA GAGCGAAACC GGGCGGCCGG AGTACATCGA
3001	GATCGAGCTA GCTGATTGGA TGTACCGCGA GATCACAGAA GGCAAGAACC
3051	CGGACGTGCT GACGGTTCAC CCCGATTACT TTTTGATCGA TCCCGGCATC
3101	GGCCGTTTTC TCTACCGCCT GGCACGCCGC GCCGCAGGCA AGGCAGAAGC
3151	CAGATGGTTG TTCAAGACGA TCTACGAACG CAGTGGCAGC GCCGGAGAGT
3201	TCAAGAAGTT CTGTTTCACC GTGCGCAAGC TGATCGGGTC AAATGACCTG
3251	CCGGAGTACG ATTTGAAGGA GGAGGCGGGG CAGGCTGGCC CGATCCTAGT
3301	CATGCGCTAC CGCAACCTGA TCGAGGGCGA AGCATCCGCC GGTTCCTAAT
3351	GTACGGAGCA GATGCTAGGG CAAATTGCCC TAGCAGGGGA AAAAGGTCGA
3401	AAAGGTCTCT TTCCTGTGGA TAGCACGTAC ATTGGGAACC CAAAGCCGTA
3451	CATTGGGAAC CGGAACCCGT ACATTGGGAA CCCAAAGCCG TACATTGGGA
3501	ACCGGTCACA CATGTAAGTG ACTGATATAA AAGAGAAAAA AGGCGATTTT
3551	TCCGCCTAAA ACTCTTTAAA ACTTATTAAA ACTCTTAAAA CCCGCCTGGC
3601	CTGTGCATAA CTGTCTGGCC AGCCCACAGC CGAAGAGCTG CAAAAAGCGC
3651	CTACCCTTCG GTCGCTGCGC TCCCTACGCC CCGCCGCTTC GCGTCGGCCT
3701	ATCGCGGCCG CTGGCCGCTC AAAAATGGCT GGCCTACGGC CAGGCAATCT
3751	ACCAGGGCGC GGACAAGCCG CGCCGTCGCC ACTCGACCGC CGGCGCCCAC
3801	ATCAAGGCAC CCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG
3851	ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG
3901	GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG
3951	GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT ATACTGGCTT
4001	AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG
4051	TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT
4101	CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA
4151	GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
4201	GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
4251	ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT
4301	GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
4351	AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
4401	CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
4451	TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
4501	GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
4551	AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG
4601	GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
4651	CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA
4701	ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG
4751	CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
4801	CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
4851	GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
4901	GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGCATTCTAG
4951	GTACTAAAAC AATTCATCCA GTAAAATATA ATATTTTATT TTCTCCCAAT
5001	CAGGCTTGAT CCCCAGTAAG TCAAAAAATA GCTCGACATA CTGTTCTTCC
5051	CCGATATCCT CCCTGATCGA CCGGACGCAG AAGGCAATGT CATACCACTT
5101	GTCCGCCCTG CCGCTTCTCC CAAGATCAAT AAAGCCACTT ACTTTGCCAT
5151	CTTTCACAAA GATGTTGCTG TCTCCCAGGT CGCCGTGGGA AAAGACAAGT
5201	TCCTCTTCGG GCTTTTCCGT CTTTAAAAAA TCATACAGCT CGCGCGGATC
5251	TTTAAATGGA GTGTCTTCTT CCCAGTTTTC GCAATCCACA TCGGCCAGAT
5301	CGTTATTCAG TAAGTAATCC AATTCGGCTA AGCGGCTGTC TAAGCTATTC
5351	GTATAGGGAC AATCCGATAT GTCGATGGAG TGAAAGAGCC TGATGCACTC
5401	CGCATACAGC TCGATAATCT TTTCAGGGCT TTGTTCATCT TCATACTCTT
5451	CCGAGCAAAG GACGCCATCG GCCTCACTCA TGAGCAGATT GCTCCAGCCA
5501	TCATGCCGTT CAAAGTGCAG GACCTTTGGA ACAGGCAGCT TTCCTTCCAG
5551	CCATAGCATC ATGTCCTTTT CCCGTTCCAC ATCATAGGTG GTCCCTTTAT
5601	ACCGGCTGTC CGTCATTTTT AAATATAGGT TTTCATTTTC TCCCACCAGC
5651	TTATATACCT TAGCAGGAGA CATTCCTTCC GTATCTTTTA CGCAGCGGTA
5701	TTTTTCGATC AGTTTTTTCA ATTCCGGTGA TATTCTCATT TTAGCCATTT
5751	ATTATTTCCT TCCTCTTTTC TACAGTATTT AAAGATACCC CAAGAAGCTA
5801	ATTATAACAA GACGAACTCC AATTCACTGT TCCTTGCATT CTAAAACCTT
5851	AAATACCAGA AAACAGCTTT TTCAAAGTTG TTTTCAAAGT TGGCGTATAA
5901	CATAGTATCG ACGGAGCCGA TTTTGAAACC GCGGTGATCA CAGGCAGCAA
5951	CGCTCTGTCA TCGTTACAAT CAACATGCTA CCCTCCGCGA GATCATCCGT
6001	GTTTCAAACC CGGCAGCTTA GTTGCCGTTC TTCCGAATAG CATCGGTAAC
6051	ATGAGCAAAG TCTGCCGCCT TACAACGGCT CTCCCGCTGA CGCCGTCCCG
6101	GACTGATGGG CTGCCTGTAT CGAGTGGTGA TTTTGTGCCG AGCTGCCGGT
6151	CGGGGAGCTG TTGGCTGGCT GGTGGCAGGA TATATTGTGG TGTAAACAAA
6201	TTGACGCTTA GACAACTTAA TAACACATTG CGGACGTTTT TAATGTACTG
6251	AATTAACGCC GAATTAATTC GGGGGATCTG GATTTTAGTA CTGGATTTTG
6301	GTTTTAGGAA TTAGAAATTT TATTGATAGA AGTATTTTAC AAATACAAAT
6351	ACATACTAAG GGTTTCTTAT ATGCTCAACA CATGAGCGAA ACCCTATAGG
6401	AACCCTAATT CCCTTATCTG GGAACTACTC ACACATTTTT ATGGAGAAAC
6451	TCGAGTTAAC CCTGAGACTG TTGGACAGAG CTCATTGGTA CCAGGAACAG
6501	GTGGTGGCGG CCCTCGGTGC GCTCGTACTG CTCCACGATG GTGTAGTCCT
6551	CGTTGTGGGA GGTGATGTCC AGCTTGGCGT CCACGTAGTA GTAGCCGGGC
6601	AGCTGCACGG GCTTCTTGGC CATGTAGATG GACTTGAACT CCACCAGGTA
6651	GTGGCCGCCG TCCTTCAGCT TCAGGGCCTT GTGGGTCTCG CCCTTCAGCA
6701	CGCCGTCGCG GGGGTACAGG CGCTCGGTGG AGGCCTCCCA GCCCATGGTC
6751	TTCTTCTGCA TCACGGGGCC GTCGGAGGGG AAGTTCACGC CGATGAACTT
6801	CACCTTGTAG ATGAAGCAGC CGTCCTGCAG GGAGGAGTCC TGGGTCACGG
6851	TCGCCACGCC GCCGTCCTCG AAGTTCATCA CGCGCTCCCA CTTGAAGCCC
6901	TCGGGGAAGG ACAGCTTCTT GTAGTCGGGG ATGTCGGCGG GGTGCTTCAC
6951	GTACACCTTG GAGCCGTACT GGAACTGGGG GGACAGGATG TCCCAGGCGA
7001	AGGGCAGGGG GCCGCCCTTG GTCACCTTCA GCTTCACGGT GTTGTGGCCC
7051	TCGTAGGGGC GGCCCTCGCC CTCGCCCTCG ATCTCGAACT CGTGGCCGTT
7101	CACGGTGCCC TCCATGCGCA CCTTGAAGCG CATGAACTCG GTGATGACGT
7151	TCTCGGAGGA GGCCATTTTG GTAGACTCGA GAGAGATAGA TTTGTAGAGA
7201	GAGACTGGTG ATTTCAGCGT GTCCTCTCCA AATGAAATGA ACTTCCTTAT
7251	ATAGAGGAAG GTCTTGCGAA GGATAGTGGG ATTGTGCGTC ATCCCTTACG
7301	TCAGTGGAGA TATCACATCA ATCCACTTGC TTTGAAGACG TGGTTGGAAC
7351	GTCTTCTTTT TCCACGATGC TCCTCGTGGG TGGGGGTCCA TCTTTGGGAC
7401	CACTGTCGGC AGAGGCATCT TGAACGATAG CCTTTCCTTT ATCGCAATGA
7451	TGGCATTTGT AGGTGCCACC TTCCTTTTCT ACTGTCCTTT TGATGAAGTG
7501	ACAGATAGCT GGGCAATGGA ATCCGAGGAG GTTTCCCGAT ATTACCCTTT
7551	GTTGAAAAGT CTCAATAGCC CTTTGGTCTT CTGAGACTGT ATCTTTGATA
7601	TTCTTGGAGT AGACGAGAGT GTCGTGCTCC ACCATGTTAT CACATCAATC
7651	CACTTGCTTT GAAGACGTGG TTGGAACGTC TTCTTTTTCC ACGATGCTCC
7701	TCGTGGGTGG GGGTCCATCT TTGGGACCAC TGTCGGCAGA GGCATCTTGA
7751	ACGATAGCCT TTCCTTTATC GCAATGATGG CATTTGTAGG TGCCACCTTC
7801	CTTTTCTACT GTCCTTTTGA TGAAGTGACA GATAGCTGGG CAATGGAATC
7851	CGAGGAGGTT TCCCGATATT ACCCTTTGTT GAAAAGTCTC AATAGCCCTT
7901	TGGTCTTCTG AGACTGTATC TTTGATATTC TTGGAGTAGA CGAGAGTGTC
7951	GTGCTCCACC ATGTTGGCAA GCTGCTCTAG CCAATACGCA AACCGCCTCT
8001	CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG
8051	ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT
8101	CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG
8151	TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG
8201	ATTACGAATT CAGGTACCAT TTAAATCCTG CAGGGTTTAA ACAGTGTTTT
8251	ACTCCTCATA TTAACTTCGG TCATTAGAGG CCACGATTTG ACACATTTTT
8301	ACTCAAAACA AAATGTTTGC ATATCTCTTA TAATTTCAAA TTCAACACAC
8351	AACAAATAAG AGAAAAAACA AATAATATTA ATTTGAGAAT GAACAAAAGG
8401	ACCATATCAT TCATTAACTC TTCTCCATCC ATTTCCATTT CACAGTTCGA
8451	TAGCGAAAAC CGAATAAAAA ACACAGTAAA TTACAAGCAC AACAAATGGT
8501	ACAAGAAAAA CAGTTTTCCC AATGCCATAA TACTCGAACG GCGCGCCTCA
8551	GCCCATATGC AGGCCGCCGT TGAGCGAGAA GTCGGCGCCG GTCGAGAAAC
8601	CGGACTCCTC CGACGACAAC CAGGCGCAGA TCGAGGCGAT CTCTTCCGGC
8651	AGGCCCAGGC GCTTGACCGG GATCGTCGCG ACGATCTTGT CGAGCACGTC
8701	CTGGCGGATC GCCTTGACCA TGTCGGTGGC GATATAGCCC GGAGAGACCG
8751	TGTTGACGGT CACGCCCTTG GTCGCCACTT CCTGCGCCAG TGCCATGGTG
8801	AAGCCATGCA CGCCGGCCTT GGCGGTGGAG TAGTTGGTCT GGCCGAACTG
8851	GCCCTTCTGC CCGTTCACCG ACGAGATGTT GACGATGCGG CCCCAGCCAC
8901	GGTCGGCCAT GCCGTCGATC ACCTGCTTGG TGACGTTGAA CAGCGAGGTC
8951	AGGTTGGTGT CGATCACCGC ATCCCAGTCG GCGCGGGTCA TCTTGCGGAA
9001	CACCACGTCG CGGGTGATAC CGGCGTTGTT GATCAGCACA TCAACCTCGC
9051	CGACCTCGGA CTTGACCTTG TCGAATGCGG TCTTGGTCGA GTCCCAGTCA
9101	GCCACATTGC CTTCCGAGGC AATGAAATCG AAGCCCAGGG CCTTCTGCTG
9151	CTCCAGCCAC TTTTCGCGGC GCGGCGAGTT GGGGCCGCAA CCGGCCACCA
9201	CACGAAAGCC ATCCTTGGCC AGCCGCTGGC AAATGGCGGT TCCGATACCA
9251	CCCATGCCGC CGGTCACATA CGCAATGCGC TGAGTCACTC TAGAATCTCT
9301	CGTCAATGGT GGCAAATAGG AAAGAGTCTC AAACTTCTTC TTTCCAATTG
9351	GAGGCCACAC CTGCATGCAC TTTACTCTTC CACCATTGCT TGTAATGGAA
9401	GTAATGTCAG TGTTGACCTT CTTCACTGGG AATCCAGTCA TGGATTTGAG
9451	GCCGCCGAAT GGAGCCACTG CGGCGGATTG CCCCCTAGAG GCACGGCTGA
9501	CTGTTGTCAC AGCGGAAGAG GATATCATAG AAGCCATTTT ACTAGTAAGA
9551	AGCTGAAAAT ATCAAAAGAA GGAACAGTCA TTAATCTATT GCATGTACTA
9601	GATTTTAGAT ATGAGTGGTC AAAAAAAACT TACGTTAATA ACGATGAAGA
9651	AGACAATGAT CCTCAGCACA ATCTCTCTCT CTCTCTCTTG GCTTCTCTTC
9701	TGGTGAATAG CACGAGAGAG GGTTTAAATG GAAGGCTCGT GGGTCCAAAA
9751	TGGGTGGCGG AGGAAATAGG AGAAGTAGGC AGTGACAAGT AATGTAGTAT
9801	TTAGTATTTG ATGAATGACA CATTTTCATT TCAGCATCAT CACCAACCAT
9851	CCTTTTGTTC CTTTGCTTCA ACTGTCACTT TCAATTGACA AAATTTTTTA
9901	TGTTTTCATG AGAAAACTAA ATTCTTATAA AGATTCATCT TCTTGAGTAT
9951	TATACGTGTA GTTTATGAAC AACACGTGTT GTTCCTATAT TTTTGTTCTG
10001	TTACCTCTAG AATAAAGTTG TCACCATTTC ATGAGTTCAA TTTTTCTTTA
10051	ATAGCCCCAA AAACAAAAGA TGATTCACAA GAAAGATGCG AATATTTTGC
10101	TATGAATCTT TTCTTAAGAG AAGCAATTAC ATTTTCACAA TAAAATTAGA
10151	TCCACGACTT AACCTAGTTT ATGTTGATTA TTTCTAGTGT TAGTATTAAG
10201	CAAAAATAAA ACTTATGAAT ACGAAGGCCT TTAAAGGAAA CTAAAGAAAG
10251	GACAAGGTAT AAACGTCCTA GAAAGTTCTA GGGTTTAGGC TTAGGGTCTA
10301	AGATATATGC TTTGAGTTTT ATGGCTTAGT AACACATTTT TGTAACACTT
10351	CTTTGTAACA TTTCTTGATA TGTTGGAGAA GTAACTCGTC TGGACAATAG
10401	TTATTTCCAA TATATAGGAA AAACGGCCTA AACAATAGCC GACGGGGACA
10451	AATACATCAT AAACAAAAAA TCCCGGTTAC AAACTTCCTA AAAAGCCATT
10501	CGGTCCACTC CGTTAAGCCT GAACTGTGCC TCCGTTATGC AAAAACGCCG
10551	TTGACCATCC GTAACCTAGT TGACTGACGG ATTATGGATT TAATCCGTTT
10601	TAAGGCCGTT AATAACACCA AAACGACGTC GTTTTGGTGT TTAAATTTTT
10651	TTTAACAACA ATTAAACCAA ACGACGTCGT TTTGGTTTAA TTAAATTTTT
10701	TTATCAAAAA CCCAAGCCCA AGCCCAAAAC TCTTAACAAA AGATAAAGCC
10751	CATCTCTATT TTTTCTAATT AAAACGCACA GCATTATGTT TCTTCTCTAA
10801	CGGATATATT TTCAATCTCA TAAATTGGGG ATTAGGGTTC TTATTTCCCA
10851	ATTCTCAATC TCTCAAAATT CTCCAAAATT CTCTGAAATT GATAATGCCT
10901	TCTTCTTCTT CAAACTCGTT TTTCTCTTTT GACAGTGAGC TTGAAGATGA
10951	TAACCATCGT GGTTTTCCTA AGACCTGTCG ATTTGGATGT CGTGTTGTGA
11001	TCAGAACCTC AAGAACTCCA AAAAACCTAG GTAGATTATT CCATACCTGT
11051	GAGAAAAATT TCAAAAGAGG AGGATTCCAC ACCTGGAAGT GGACTGATGT
11101	GTCTTTAGTA GAAGAAGTAG AGGACATAAA GGCTTACATT CATAACCGTG
11151	AGAAGTGTCA CGATGAAGAA ATGTTATTAT TGAAGGCTCA GATTCGTGGC
11201	TGTGAGAAGA TGATTGAAGG CTTGAAAGGA GAAGCAAAAC GTATGAAGCT
11251	AATTGTTGTT GCCGGAATAG TTGTGTTTGG TTGCTTTTTG TGTCTCTCTA
11301	AGTGATGTAT GAGATGAATG TTTGTGTATG TGATGTTGTT TTGTCTCAAT
11351	AATTAGTCAC TGATGTTGTA TGTAATGTTG TGTTTTGCAT CTCTAATTAG
11401	TTAATAATGA ATGTTGTTCT TATGTAATGT TTGATTTAAT CAATGGCTTT
11451	TGCAAATAAA TCCATAACAG AACNTATTCA ATATTTTCGA AAACATAACA
11501	AAGGTTTCAA AAGAAATTGC ATGTTGATTA GCTGAGTTTT CAAACAAAAT
11551	GCATTACATA GACAGACCCT GCTTCATAAT CCCCAAAACA CAAAAGAGAA
11601	GCATGCTAAT AACCGCAACT AATATCCAAA GACAGCTTCA TAATCCCAAA
11651	ACACAAAAAA AGAAGATTCA TAACCGATCC TTCATGTATT TAAAGAAAAT
11701	CAGACAACAA GCAAAGACTT AATCTTCCTG AGTAACTGAT GAGCTCAAGT
11751	CGACGTTTAA ACAGTGTTTT ACTCCTCATA TTAACTTCGG TCATTAGAGG
11801	CCACGATTTG ACACATTTTT ACTCAAAACA AAATGTTTGC ATATCTCTTA
11851	TAATTTCAAA TTCAACACAC AACAAATAAG AGAAAAAACA AATAATATTA
11901	ATTTGAGAAT GAACAAAAGG ACCATATCAT TCATTAACTC TTCTCCATCC
11951	ATTTCCATTT CACAGTTCGA TAGCGAAAAC CGAATAAAAA ACACAGTAAA
12001	TTACAAGCAC AACAAATGGT ACAAGAAAAA CAGTTTTCCC AATGCCATAA
12051	TACTCGAACT ACGTATTATT TGCGCTCGAC TGCCAGCGCC ACGCCCATGC
12101	CGCCGCCGAT GCACAGCGAG GCCAGGCCCT TCTTCGCGTC ACGGCGCTTC
12151	ATCTCGTGCA GCAGCGTCAC CAGGATACGG CAGCCCGACG CGCCGATCGG
12201	GTGGCCGATG GCGATGGCGC CGCCGTTCAC ATTGACCTTG GAGGTGTCCC
12251	AGCCCATCTG CTGGTGCACC GCCAGCGCCT GCGCGGCAAA GGCCTCGTTG
12301	ATCTCCATCA GGTCCAGGTC TTGCGGGGTC CACTCGGCGC GCGACAGGGC
12351	GCGCTTGGAG GCCGGCACCG GGCCCATGCC CATCACCTTG GGATCGACAC
12401	CGGCGTTGGC ATAGCTCTTG ATCGTGGCCA GCGGGGTCAG GCCCAGTTCC
12451	TTGGCCTTGG CCGCCGACAT CACCACCACC GCGGCGGCGC CGTCGTTCAG
12501	GCCCGAGGCG TTGGCCGCGG TCACCGTGCC GGCCTTGTCG AAGGCGGGCT
12551	TGAGGCCGGA CATGCTGTCC AGCGTGGCGC CCTGGCGCAC GAACTCGTCG
12601	GTCTTGAAGG CCACCGGGTC GCCCTTGCGC TGCGGGATCA GCACCGGGAC
12651	GATCTCTTCG TCAAACTTGC CGGCCTTCTG CGCGGCTTCG GCCTTGTTCT
12701	GCGAGCCGAC GGCGAACTCA TCCTGCGCCT CGCGTGTGAT GCCGTATTCC
12751	TTGGCCACGT TCTCGGCGGT GATGCCCATG TGGTACTGGT TGTACACGTC
12801	CCACAGGCCG TCGACGATCA TGGTGTCGAC CAGCTTGGCA TCGCCCATGC
12851	GGAAACCATC GCGCGAGCCC GGCAGCACGT GCGGGGCGGC GCTCATGTTT
12901	TCCTGGCCGC CGGCCACCAC GATCTCGGCG TCGCCCGCCA TGATCGCGTT
12951	GGCGGCCAGC ATCACGGCCT TCAGGCCCGA GCCGCACACC TTGTTGATGG
13001	TCATGGCCGG CACCATCGCC GGCAGGCCGG CCTTGATCGC GGCCTGGCGT
13051	GCGGGGTTCT GGCCCGAACC GGCGGTCAGC ACCTGGCCCA TGATGACTTC
13101	GCTCACCTGC TCCGGCTTGA CGCCGGCGCG CGCCGGCGCG GCCTTGATGA
13151	CCACGGCACC CAGTTCCGGT GCGGGGTTCT TGGCCAGCGA GCCGCCAAAC
13201	TTGCCGACCG CGGTGCGGGC GGCGGATACG ATGACAACGT CAGTCACTCT
13251	AGAATCTCTC GTCAATGGTG GCAAATAGGA AAGAGTCTCA AACTTCTTCT
13301	TTCCAATTGG AGGCCACACC TGCATGCACT TTACTCTTCC ACCATTGCTT
13351	GTAATGGAAG TAATGTCAGT GTTGACCTTC TTCACTGGGA ATCCAGTCAT
13401	GGATTTGAGG CCGCCGAATG GAGCCACTGC GGCGGATTGC CCCCTAGAGG
13451	CACGGCTGAC TGTTGTCACA GCCGAACAGG ATATCATAGA AGCCATTTTG
13501	GATCCAAGAA GCTGAAAATA TCAAAAGAAG GAACAGTCAT TAATCTATTG
13551	CATGTACTAG ATTTTAGATA TGAGTGGTCA AAAAAAACTT ACGTTAATAA
13601	CGATGAAGAA GACAATGATC CTCAGCACAA TCTCTCTCTC TCTCTCTTGG
13651	CTTCTCTTCT GGTGAATAGC ACGAGAGAGG GTTTAAATGG AAGGCTCGTG
13701	GGTCCAAAAT GGGTGGCGGA GGAAATAGGA GAAGTAGGCA GTGACAAGTA
13751	ATGTAGTATT TAGTATTTGA TGAATGACAC ATTTTCATTT CAGCATCATC
13801	ACCAACCATC CTTTTGTTCC TTTGCTTCAA CTGTCACTTT CAATTGACAA
13851	AATTTTTTAT GTTTTCATGA GAAAACTAAA TTCTTATAAA GATTCATCTT
13901	CTTGAGTATT ATACGTGTAG TTTATGAACA ACACGTGTTG TTCCTATATT
13951	TTTGTTCTGT TACCTCTAGA ATAAAGTTGT CACCATTTCA TGAGTTCAAT
14001	TTTTCTTTAA TAGCCCCAAA AACAAAAGAT GATTCACAAG AAAGATGCGA
14051	ATATTTTGCT ATGAATCTTT TCTTAAGAGA AGCAATTACA TTTTCACAAT
14101	AAAATTAGAT CCACGACTTA ACCTAGTTTA TGTTGATTAT TTCTAGTGTT
14151	AGTATTAAGC ACAAATAAGA CTTATGAATA CGAAGGCCTT TAAAGGAAAC
14201	TAAAGAAAGG ACAAGGTATA AACGTCCTAG AAAGTTCTAG GGTTTAGGCT
14251	TAGGGTCTAA GATATATGCT TTGAGTTTTA TGGCTTAGTA ACACATTTTT
14301	GTAACACTTC TTTGTAACAT TTCTTGATAT GTTGGAGAAG TAACTCGTCT
14351	GGACAATAGT TATTTCCAAT ATATAGGAAA AACTTCCTAA ACAATAGCCG
14401	ACGGGGACAA ATACATCATA AACAAAAGAT CCCGGTTACA AACTTCCTAA
14451	AAAGCCATTC GGTCCACTCC GTTAAGCCTG AACTGTGCCT CCGTTATGCA
14501	AAAACGCCGT TGACCATCCG TAACCTAGTT GACTGACGGA TTATGGATTT
14551	AATCCGTTTT AAGGCCGTTA ATAACACCAA AACGACGTCG TTTTGGTGTT
14601	TTAATTTTTT TTAACAACAA TTAAACCAAA CGACGTCGTT TTGGTTTAAT
14651	TAAATTTTTT TATCAAAAAC CCAAGCCCAA GCCCAAAACT CTTAACAAAA
14701	GATAAAGCCC ATCTCTATTT TTTCTAATTA AAACGCACAG CATTATGTTT
14751	CTTCTCTAAC GGATATATTT TCAATCTCAT AAATTGGGGA TTAGGGTTCT
14801	TATTTCCCAA TTCTCAATCT CTCAAAATTC TCCAAAATTC TCTGAAATTG
14851	ATAATGCCTT CTTCTTCTTC AAACTCGTTT TTCTCTTTTG ACAGTGAGCT
14901	TGAAGATGAT AACCATCGTG GTTTTCCTAA GACCTGTCGA TTTGGATGTC
14951	GTGTTGTGAT CAGAACCTCA ATAACACCAA AAAACCTAGG TAGATTATTC
15001	CATACCTGTG AGAAAAATTT CAAAAGAGGA GGATTCCACA CCTGGAAGTG
15051	GACTGATGTG TCTTTAGTAG AAGAAGTAGA GGACATAAAG GCTTACATTC
15101	ATAACCGTGA GAAGTGTCAC GATGAAGAAA TGTTATTATT GAAGGCTCAG
15151	ATTCGTGGCT GTGAGAAGAT GATTGAAGGC TTGAAAGGAG AAGCAAAACG
15201	TATGAAGCTA ATTGTTCTTG CCGGAATAGT TGTGTTTGGT TGCTTTTTGT
15251	GTCTCTCTAA GTGATGTATG AGATGAATGT TTGTGTATGT GATGTTGTTT
15301	TGTCTCAATA ATTAGTCACT GATGTTGTAT GTAATGTTGT GTTTTGCATC
15351	TCTAATTAGT TAATAATGAA TGTTGTTCTT ATGTAATGTT TGATTTAATC
15401	AATGGCTTTT GCAAATAAAT CCATAACAGA ACNTATTCAA TATTTTCGAA
15451	AACATAACAA AGGTTTCAAA AGAAATTGCA TTAGCATTAG CTGAGTTTTC
15501	AAACAAAATG CATTACATAG ACAGACCCTG CTTCATAATC CCCAAAACAC
15551	AAAAGAGAAG CATGCTAATA ACCGCAACTA ATATCCAAAG ACAGCTTCAT
15601	AATCCCAAAA CACAAAAAAA GAAGATTCAT AACCGATCCT TCATGTATTT
15651	AAAGAAAATC AGACAACAAG CAAAGACTTA ATCTTCCTGA GTAATGGAAG
15701	AGCTCAACTG CAGGTTTAAA CAGTGTTTTA CTCCTCATAT TAACTTCGGT
15751	CATTAGAGGC CACGATTTGA CACATTTTTA CTCAAAACAA AATGTTTGCA
15801	TATCTCTTAT AATTTCAAAT TCAACACACA ACAAATAAGA GAAAAAACAA
15851	ATAATATTAA TTTGAGAATG AACAAAAGGA CCATATCATT CATTAACTCT
15901	TCTCCATCCA TTTCCATTTC ACAGTTCGAT AGCGAAAACC GAATAAAAAA
15951	CACAGTAAAT TACAAGCACA ACAAATGGTA CAAGAAAAAC AGTTTTCCCA
16001	ATGCCATAAT ACTCGAACGC GATCGCTCAG CCCTTGGCTT TGACGTAACG
16051	GCCGGGCGCC GCCTCGATCG CGGTGTAGCG GGCGTTGCCG GGCTTGGCCT
16101	TGGGCTTGAC CTTCTTGCCG CCATGCTGGG TCAGGAACCC GGCCCATTGC
16151	GGCCACCAGC TGCCCGGCAC TTCCTGCGCG CCATCGAACC AGGCCTGGGC
16201	ATCGGCGGCG CCACCGTCGT TGATCCAGTA GCTGCGCTTG TTCTTGGCCA
16251	CCGAGTTGAT CACGCCGGCG ATATGGCCGG ACGCGCCCAG CACGAAGCGG
16301	TTGGCGCCCG GCTTGCCCTG GTTGAGGATG TCGAGCGAAC CGTACGCCGA
16351	CATCCACGGC ACGATGTGGT CTTCGCGCGA ACCGTAGATG AAGGCCGGGG
16401	CGTCGATCAG GCCGAGGTCG ATCTTTTCGC CGGCCACCGT CAGCTTGCCC
16451	GGCACTTTCA GGCTGTTTTC CAGGTAGGTG TTGCGCAGGT ACCAGCAGAA
16501	CATCGGGCCC GGCAAATTGG TGCTGTCCGA ATTCCAGAAC AGCAGGTCAA
16551	ACGCCGCCGG CTCATTGCCT TTGAGGTAGT TCGACTGCAC ATAGTTCCAT
16601	ACCAGGTCGT TCGGACGCAG GCTCGAGAAG GTCGAGGCCA GGTCACGGCC
16651	CGGCATCAGG CCGCCATCGC GCAATTGCTG TTCACGCAGC GCGACCTGGG
16701	TTTCATCGAC GAAGACGTCG AGCACGCCGG TGTCGCTGAA GTCGAGGAAG
16751	GTGGTCAGCA GGGTCAGGCT GGCCGCCGGG TGCTGGCCAC GCGCCGCCAG
16801	TACCGCCAGT GCGGTGGCAA CGATGGTGCC GCCCACGCAG AAGCCGAACA
16851	TGTTCAGCTT GTCCTGGCCG CTGACGTCCT GGACGATGCG GATCGCTTCG
16901	ATCACGCCCT GCTCCACGTA GTCGTCCCAG GTGGTGCCGG CCAGCGACTT
16951	GTCCGGATTG CTCCACGAGA TCAGGAACAC GGTGTTGCCC TGCTCCACCG
17001	CGTAGCGCAC CAGCGAATTT TCCGGTTGCA GGTCGAGGAT GTAGAACTTG
17051	TTGATGCACG GCGGCACCAT CAACAGCGGG CGCTGGCTGA CCGTCGGCGT
17101	GGTCGGCGTG TACTGGATCA GCTGGAACAG CGGATTTTCG TAAATCACGG
17151	TGCCCGGGGT AATGGCCAGG TTGCGGCCCA CTTCAAAGGC CGATTCGTCC
17201	GACAGCGAGA TATGGCCCTT GTTGATATCG CCCAGCATAT TGACCAGGCC
17251	ACGCGTCAGG CTCTCGCCCT TGGTTTCAAT CAGTTTTTGC TGCGCTTCCG
17301	GGTTGGTGGC GAGGAAGTTC GCGGGCGACA TGGCATCAAT CACCTGCTGC
17351	ACGGCAAAGC GTATTTTCTG CTTTTGCTGG GGTGCGGTGT CCACCGCCTC
17401	CACCATGGCA CTGAGGAATT TGGCGTTGAG CAGGTAAGAT GCGGCATTGA
17451	AGGCCGACAT CGGATTGCCC TGCCAGGCTG CCGAGCTGAA GCGGCGGTCG
17501	CTGACGGCTG GCGCCTTGCC AGCCAAAAAA TCCTGCCACA ACGCGGTGAA
17551	GTCACGCAGA TAATCGTTTT TCAGCTGCTC CATCGCTTCC GGTTTGAGCG
17601	CAACGCCGAT ATCCTGCAAC ATGGTGGCCA TCGGGTTCGC CTCGGTGGTG
17651	GGCGCCTTGC TGAACCAGGA TTGCCACTGC AGCTCATCGT TGTTCTTGTT
17701	ACTCACTCTA GAATCTCTCG TCAATGGTGG CAAATAGGAA AGAGTCTCAA
17751	ACTTCTTCTT TCCAATTGGA GGCCACACCT GCATGCACTT TACTCTTCCA
17801	CCATTGCTTG TAATGGAAGT AATGTCAGTG TTGACCTTCT TCACTGGGAA
17851	TCCAGTCATG GATTTGAGGC CGCCGAATGG AGCCACTGCG GCGGATTGCC
17901	CCCTAGAGGC ACGGCTGACT GTTGTCACAG CGGAAGAGGA TATCATAGAA
17951	GCCATTTTTG TACAAAGAAG CTGAAAATAT CAAAAGAAGG AACAGTCATT
18001	AATCTATTGC ATGTACTAGA TTTTAGATAT GAGTGGTCAA AAAAAACTTA
18051	CGTTAATAAC GATGAAGAAG ACAATGATCC TCAGCACAAT CTCTCTCTCT
18101	CTCTCTTGGC TTCTCTTCTG GTGAATAGCA CGAGAGAGGG TTTAAATGGA
18151	AGGCTCGTGG GTCCAAAATG GGTGGCGGAG GAAATAGGAG AAGTAGGCAG
18201	TGACAAGTAA TGTAGTATTT AGTATTTGAT GAATGACACA TTTTCATTTC
18251	AGCATCATCA CCAACCATCC TTTTGTTCCT TTGCTTCAAC TGTCACTTTC
18301	AATTGACAAA ATTTTTTATG TTTTCATGAG AAAACTAAAT TCTTATAAAG
18351	ATTCATCTTC TTGAGTATTA TACGTGTAGT TTATGAACAA CACGTGTTGT
18401	TCCTATATTT TTGTTCTGTT ACCTCTAGAA TAAAGTTGTC ACCATTTCAT
18451	GAGTTCAATT TTTCTTTAAT AGCCCCAAAA ACAAAAGATG ATTCACAAGA
18501	AAGATGCGAA TATTTTGCTA TGAATCTTTT CTTAAGAGAA GCAATTACAT
18551	TTTCACAATA AAATTAGATC CACGACTTAA CCTAGTTTAT GTTGATTATT
18601	TCTAGTGTTA GTATTAAGCA AAAATAAAAC TTATGAATAC GAAGGCCTTT
18651	AAAGGAAACT AAAGAAAGGA CAAGGTATAA ACGTCCTAGA AAGTTCTAGG
18701	GTTTAGGCTT AGGGTCTAAG ATATATGCTT TGAGTTTTAT GGCTTAGTAA
18751	CACATTTTTG TAACACTTCT TTGTAACATT TCTTGATATG TTGGAGAAGT
18801	AACTCGTCTG GACAATAGTT ATTTCCAATA TATAGGAAAA ACGGCCTAAA
18851	CAATAGCCGA CGGGGACAAA TACATCATAA ACAAAAAATC CCGGTTACAA
18901	ACTTCCTAAA AAGCCATTCG GTCCACTCCG TTAAGCCTGA ACTGTGCCTC
18951	CGTTATGCAA AAACGCCGTT GACCATCCGT AACCTAGTTG ACTGACGGAT
19001	TATGGATTTA ATCCGTTTTA AGGCCGTTAA TAACACCAAA ACGACGTCGT
19051	TTTGGTGTTT TAATTTTTTT TAACAACAAT TAAACCAAAC GACGTCGTTT
19101	TGGTTTAATT AAATTTTTTT ATCAAAAACC CAAGCCCAAG CCCAAAACTC
19151	TTAACAAAAG ATAAAGCCCA TCTCTATTTT TTCTAATTAA AACGCACAGC
19201	ATTATGTTTC TTCTCTAACG GATATATTTT CAATCTCATA AATTGGGGAT
19251	TAGGGTTCTT ATTTCCCAAT TCTCAATCTC TAACACTTCT CCAAAATTCT
19301	CTGAAATTGA TAATGCCTTC TTCTTCTTCA AACTCGTTTT TCTCTTTTGA
19351	CAGTGAGCTT GAAGATGATA ACCATCGTGG TTTTCCTAAG ACCTGTCGAT
19401	TTGGATGTCG TGTTGTGATC AGAACCTCAA GAACTCCAAA AAACCTAGGT
19451	AGATTATTCC ATACCTGTGA GAAAAATTTC AAAAGAGGAG GATTCCACAC
19501	CTGGAAGTGG ACTGATGTGT CTTTAGTAGA AGAAGTAGAG GACATAAAGG
19551	CTTACATTCA TAACCGTGAG AAGTGTCACG ATGAAGAAAT GTTATTATTG
19601	AAGGCTCAGA TTCGTGGCTG TGAGAAGATG ATTGAAGGCT TGAAAGGAGA
19651	AGCAAAACGT ATGAAGCTAA TTGTTGTTGC CGGAATAGTT GTGTTTGGTT
19701	GCTTTTTGTG TCTCTCTAAG TGATGTATGA GATGAATGTT TGTGTATGTG
19751	ATGTTGTTTT GTCTCAATAA TTAGTCACTG ATGTTGTATG TAATGTTGTG
19801	TTTTGCATCT CTAATTAGTT AATAATGAAT GTTGTTCTTA TGTAATGTTT
19851	GATTTAATCA ATGGCTTTTG CAAATAAATC CATAACAGAA CNTATTCAAT
19901	ATTTTCGAAA ACATAACAAA GGTTTCAAAA GAAATTGCAT TAGCATTAGC
19951	TGAGTTTTCA AACAAAATGC ATTACATAGA CAGACCCTGC TTCATAATCC
20001	CCAAAACACA AAAGAGAAGC ATGCTAATAA CCGCAACTAA TATCCAAAGA
20051	CAGCTTCATA ATCCCAAAAC ACAAAAAAAG AAGATTCATA ACCGATCCTT
20101	CATGTATTTA AAGAAAATCA GACAACAAGC AAAGACTTAA TCTTCCTGAG
20151	TAACTGATGA GCTCAAAAGC TTGGCACTGG CCGTCGTTTT ACAACGTCGT
20201	GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC
20251	CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT
20301	CCCAACAGTT GCGCAGCCTG AATGGCGAAT GCTAGAGCAG CTTGAGCTTG
20351	GATCAGATTG TCGTTTCCCG CCTTCAGTTT AAACTATCAG TGTTTGACAG
20401	GATATATTGG CGGGTAAACC TAAGAGAAAA GAGCGTTTAT TAGAATAACG
20451	GATATTTAAA AGGGCGTGAA AAGGTTTATC CGTTCGTCCA TTTGTATGTG