109025 ⎘
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor; Recombinant DNA-technology; Processes for the isolation, preparation or purification of DNA or RNA; Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
COMPOSITIONS AND METHODS FOR HIGH FIDELITY ASSEMBLY OF NUCLEIC ACIDS
#2NUCLEIC ACID BASED DATA STORAGE
#3Nucleic acid based data storage
#4METHODS FOR IN VITRO JOINING AND COMBINATORIAL ASSEMBLY OF NUCLEIC ACID MOLECULES
#5ω-Transaminase mutant obtained by DNA synthetic shuffling combined mutation and use
#6A LIBRARY OF POLYNUCLEOTIDES
#7Nucleic acid based data storage
#8COMPOSITIONS AND METHODS FOR SYNTHETIC GENE ASSEMBLY
#9Compositions and methods for high fidelity assembly of nucleic acids
#10Yeast Cell Extract Assisted Construction of DNA Molecules
#11Compositions and methods for evading bacterial defense mechanisms
#12GM-CSF and IL-4 Conjugates, Compositions, and Methods Related Thereto
#13Generation of synthetic genomes
#14Gene Synthesis by Self-Assembly of Small Oligonucleotide Building Blocks
#15ASSEMBLY AND ERROR REDUCTION OF SYNTHETIC GENES FROM OLIGONUCLEOTIDES
#16LIBRARIES OF NUCLEIC ACIDS AND METHODS FOR MAKING THE SAME
#17SELF-DIVERSIFYING SYSTEMS AND METHODS OF USE THEREOF
#18High efficient indexing method for genome assembly
#19Nucleic acid based data storage
#20Molecular fabrication
#21SYNTHETIC ATTC RECOMBINATION SITES FOR PROTEIN DOMAIN SHUFFLING
#22Method of improving characteristics of proteins
#23Genetic construct
#24Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
#25COMPOSITIONS AND METHODS FOR SYNTHETIC GENE ASSEMBLY
#26Nucleic acid based data storage
#27Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
#28PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES
#29Gene Synthesis by Self-Assembly of Small Oligonucleotide Building Blocks
#30Engineered FHA domains
#31Method of improving characteristics of proteins
#32GM-CSF and IL-4 conjugates, compositions, and methods related thereto
#33Fusion polymerase and method for using the same
#34Genetic construct
#35Polypeptide for genome shuffling in plants, and use therefor
#36Methods for Nucleic Acid Manipulation
#37Compositions and methods for polynucleotide assembly
#38Synthon Formation
#39Synthetic auxotrophs with ligand dependent essential genes for biosafety
#40Generation of synthetic genomes
#41Nucleic acid based data storage
#42Methods and materials for assembling nucleic acid constructs
#43Fusion polymerase and method for using the same
#44Rapid assembly of multiple arbitrary length DNA fragments
#45Protein variant generation by region shuffling
#46Method for Directed DNA Evolution using Combinatorial DNA Libraries
#47Methods for nucleic acid manipulation
#48Compositions and methods for synthetic gene assembly
#49Methods for nucleic acid manipulation
#50Method of preparing nucleic acid molecules
#51Molecular fabrication
#52Fusion polymerase and method for using the same
#53Synthon formation
#54Compositions and methods for creating altered and improved cells and organisms
#55Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
#56Methods for generating pools of variants of a DNA template
#57Massively parallel combinatorial genetics
#58GENE SHUFFLING METHODS
#59Methods for nucleic acid manipulation
#60Method of preparing nucleic acid molecules
#61Methods for nucleic acid manipulation
#62Methods for nucleic acid manipulation
#63Methods for nucleic acid manipulation
#64Compositions and methods for creating altered and improved cells and organisms
#65Rapid assembly of multiple arbitrary length DNA fragments
#66Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
#67DESIGN, SYNTHESIS AND ASSEMBLY OF SYNTHETIC NUCLEIC ACIDS
#68Multi-site mutagenesis
#69Method of generating gene mosaics
#70Compositions and Methods For High Fidelity Assembly of Nucleic Acids
#71Method for specifically producing a joined DNA fragment comprising a sequence derived from a target gene
#72METHODS OF GENERATING MODIFIED POLYNUCLEOTIDE LIBRARIES AND METHODS OF USING THE SAME FOR DIRECTED PROTEIN EVOLUTION
#73Methods and Materials for Nucleic Acid Manipulation
#74Microfluidic platform for synthetic biology applications
#75METHODS FOR GENERATING POLYNUCLEOTIDES HAVING DESIRED CHARACTERISTICS BY ITERATIVE SELECTION AND RECOMBINATION
#76Evolution of whole cells and organisms by recursive sequence recombination
#77ISOLATING BIOLOGICAL MODULATORS FROM BIODIVERSE GENE FRAGMENT LIBRARIES
#78COMBINATORIAL METHODS FOR OPTIMIZING ENGINEERED MICROORGANISM FUNCTION
#79Method for in Vitro Molecular Evolution of Protein Function
#80METHODS FOR IN VITRO JOINING AND COMBINATORIAL ASSEMBLY OF NUCLEIC ACID MOLECULES
#81METHODS AND COMPOSITIONS FOR POLYPEPTIDE ENGINEERING
#82Method for In Vitro Molecular Evolution of Protein Function
#83Hybrid polypeptide of a maltogenic alpha-amylase and a cyclodextrin glucanotransferase
#84USE OF RNA TRANS-SPLICING FOR ANTIBODY GENE TRANSFER AND ANTIBODY POLYPEPTIDE PRODUCTION
#85Evolution of whole cells and organisms by recursive sequence recombination
#86GENE SITE SATURATION MUTAGENESIS
#87METHOD FOR CREATING A VIRAL GENOMIC LIBRARY, A VIRAL GENOMIC LIBRARY AND A KIT FOR CREATING THE SAME
#88Polypeptides Modified by Protein Trans-Splicing Technology
#89METHOD OF OPTIMIZING CODON USAGE THROUGH DNA SHUFFLING
#90Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions
#91ISOLATING BIOLOGICAL MODULATORS FROM BIODIVERSE GENE FRAGMENT LIBRARIES
#92METHODS AND COMPOSITIONS FOR POLYPEPTIDE ENGINEERING
#93VIRAL RECOMBINEERING AND USES THEREOF
#94Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
#95Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
#96Methods for nucleic acid manipulation
#97Methods for nucleic acid manipulation
#98Methods for nucleic acid manipulation
#99Methods for nucleic acid manipulation
#100Methods for nucleic acid manipulation
#101Methods for nucleic acid manipulation
#102ISOLATING BIOLOGICAL MODULATORS FROM BIODIVERSE GENE FRAGMENT LIBRARIES
#103Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
#104Framework-Shuffling Of Antibodies
#105Preparation Method for a Protein With New Function Through Simultaneous Incorporation of Functional Elements
#106Methods and compositions for cellular and metabolic engineering
#107Hybrid polypeptide of a maltogenic alpha-amylase and a cyclodextrin glucanotransferase
#108Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules
#109Cell death pre-mRNA-encoding DNA
#110Libraries of recombinant chimeric proteins
#111Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
#112METHOD OF IN VITRO POLYNUCLEOTIDE SEQUENCES SHUFFLING BY RECURSIVE CIRCULAR DNA MOLECULES FRAGMENTATION AND LIGATION
#113NUCLEIC ACID CLONING
#114Method For Expressing Polypeptides In Eukaryotic Cells Using Alternative Splicing
#115Method for the Identification of Suitable Fragmentation Sites in a Reporter Protein
#116Method for in vitro molecular evolution of protein function
#117Assembly of large nucleic acids
#118Method for in Vitro Molecular Evolution of Protein Function
#119TRANS-SPLICING MEDIATED PHOTODYNAMIC THERAPY
#120Methods and compositions for polypeptide engineering
#121METHODS AND CELLS FOR CREATING FUNCTIONAL DIVERSITY AND USES THEREOF
#122Gene site saturation mutagenesis
#123Transgenomic mitochondria, transmitochondrial cells and organisms, and methods of making and using
#124Design, synthesis and assembly of synthetic nucleic acids
#125Libraries and their design and assembly
#126Methods for modulating cellular and organismal phenotypes
#127Method for creating polynucleotide and polypeptide sequences
#128METHODS FOR GENERATING POLYNUCLEOTIDES HAVING DESIRED CHARACTERISTICS BY ITERATIVE SELECTION AND RECOMBINATION
#129Method for in vitro molecular evolution of protein function
#130Method of DNA Shuffling with Polynucleotides Produced by Blocking or Interrupting a Synthesis or Amplification Process
#131Incrementally truncated nucleic acids and methods of making same
#132Incrementally Truncated Nucleic Acids and Methods of Making Same
#133Methods and compositions for cellular and metabolic engineering
#134Prebiotic
#135Protein Design Automation for Protein Libraries
#136PROCESS FOR GENERATING SEQUENCE-SPECIFIED PROTEASES BY DIRECTED EVOLUTION AND USE THEREOF
#137Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
#138NUCLEIC ACID MOLECULES ENCODING MISMATCH ENDONUCLEASES AND METHODS OF USE THEREOF
#139Isolating biological modulators from biodiverse gene fragment libraries
#140METHOD FOR IN VITRO MOLECULAR EVOLUTION OF PROTEIN FUNCTION
#141Method of increasing complementarity in a heteroduplex
#142Integrated systems and methods for diversity generation and screening
#143CREATION OF DIVERSITY IN POLYPEPTIDES
#144Combinatorial Biology
#145Method for the identification of suitable fragmentation sites in a reporter protein
#146Method of introducing a mitochondrial genome into a eukaryotic cell mitochondrion
#147Synthesis of error-minimized nucleic acid molecules
#148Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
#149Optimization of immunomodulatory properties of genetic vaccines
#150Optimization of immunomodulatory properties of genetic vaccines
#151Evolution of whole cells and organisms by recursive sequence recombination
#152Methods and compositions for polypeptide engineering
#153Evolution of whole cells and organisms by recursive sequence recombination
#154Lipolytic enzyme genes
#155Methods and compositions for polypeptide engineering
#156Gene synthesis using pooled DNA
#157Methods for nucleic acid manipulation
#158Methods and compositions for cellular and metabolic engineering
#159DNA shuffling to produce herbicide selective crops
#160DNA shuffling to produce herbicide selective crops
#161Methods of mixing large numbers of heterologous genes
#162Framework-shuffling of antibodies
#163Methods and compositions for polypeptide engineering
#164Nucleic acid molecules encoding endonucleases and methods of use thereof
#165Methods and compositions for polypeptide engineering
#166Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations
#167Use of RNA trans-splicing for antibody gene transfer and antibody polypeptide production
#168Screening method for identification of efficient pre-trans-splicing molecules
#169Methods for forming recombined nucleic acids
#170Non-random method of gene shuffling
#171Method of error reduction in nucleic acid populations
#172Nucleic acid cloning
#173Gene shuffling by template switching
#174Method for the repair of mutated RNA from genetically defective DNA and for the specific destruction of tumor cells by RNA trans-splicing, and a method for the detection of naturally trans-spliced cellular RNA
#175Use of spliceosome mediated RNA trans-splicing for immunotherapy
#176Methods and compositions for polypeptide engineering
#177Polygonal nanostructures of polynucleic acid multi-crossover molecules and assembly of lattices based on double crossover cohesion
#178Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
#179Multi-site mutagenesis
#180Antigen library immunization
#181Methods for nucleic acid manipulation
#182Method for in vitro molecular evolution of protein function
#183Isolating biological modulators from biodiverse gene fragment libraries
#184Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions
#185Method for in vitro molecular evolution of protein function
#186Directed evolution of proteins
#187Compositions and methods for producing libraries with controlled compositions and screening probabilities
#188Optimization of immunomodulatory properties of genetic vaccines
#189Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
#190Nucleic acid molecules encoding cel I endonuclease and methods of use thereof
#191Method of DNA shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
#192Identifying oligonucleotides for in vitro recombination
#193Nucleic acid cloning
#194Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
#195Process for the reversible functional inactivation of the mismatch repair system
#196Optimization of immunomodulatory properties of genetic vaccines
#197Method for evolving a cell having desired phenotype and evolved cells
#198Method for obtaining circular mutated or chimeric polynucleotides
#199Method of increasing complementarity in a heteroduplex
#200Method for the production of nucleic acids consisting of stochastically combined parts of source nucleic acids
#201Process for preparing variant polynucleotides
#202Process for preparing variant polynucleotides
#203Method for preparing polypeptides variants
#204Whole cell engineering by mutagenizing a substantial portion of a starting genome combining mutations and optionally repeating
#205Methods for generating cellulases
#206Method for producing a synthetic gene or other DNA sequence
#207Saturation mutagenesis in directed evolution
#208Optimization of immunomodulatory properties of genetic vaccines
#209DNA shuffling to produce herbicide selective crops
#210Methods and compositions for polypeptide engineering
#211Libraries of recombinant chimeric proteins
#212Method for preparing polypeptides variants
#213Methods for improving or altering promoter/enhancer properties